1141. protein complex purification, western shows tag protein was pulled out, but silv - (reply: 10)
1142. vortexing of antibodies upon dilution in 3% skim milk - (reply: 3)
1143. Silver staining after Coomassie stain - (reply: 7)
1144. Western Blot with Plasma - (reply: 7)
1145. Western Transfer and Detection of small protein - (reply: 6)
1146. 2D protein gel for miRNA target screening? - (reply: 2)
1147. Immunoprecipitation Question - Is it Possible to pull down a receptor by probing for its ligand (reply: 1)
1148. protein expression from glycerol stock - (reply: 2)
1149. use of Pierce stripping buffer in WB - (reply: 3)
1150. What should be the pH of the leamlli sample buffer? - (reply: 7)
1151. Streptavidin-biotin peptide pull down for silver stain - (reply: 2)
1152. anti-BRCA1 (ab-1) for WB - (reply: 8)
1153. Short peptide stability - (reply: 4)
1154. low MW bands left on gel during transfer to PVDF - (reply: 1)
1155. confusing shape of survivin protein band - (reply: 3)
1156. Abnormal polymerization of acrylamide when preparing SDS-PAGE - (reply: 9)
1157. Troubleshoot: Western; no bands appear in reduced lanes - (reply: 8)
1158. A basic knowledge for RPHPLC - (reply: 2)
1159. 2nd dimension temperture effects? - (reply: 3)
1160. Expression of an N-terminally tagged golgi protein - (reply: 1)
1161. sepharose bead - (reply: 5)
1162. glycine or tris buffer - (reply: 5)
1163. Reproducibility - (reply: 4)
1164. Native PAGE questions - (reply: 1)
1165. size of tagged proteins - (reply: 3)
1166. SDS PAGE problem - (reply: 5)
1167. Questions about IP - (reply: 2)
1168. How to detect protein kinase from lysate - (reply: 4)
1169. Which water is better for chemical preparation ? - water grade for protein purification (reply: 2)
1170. SDS Page running problem - prestained protein marker - (reply: 5)

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