1111. How to induce hydrophobic protein-protein interactions? - Experimental conditions like..? (reply: 1)
1112. suggestions to express protein in e coli - (reply: 3)
1113. protein complex purification, western shows tag protein was pulled out, but silv - (reply: 10)
1114. vortexing of antibodies upon dilution in 3% skim milk - (reply: 3)
1115. Silver staining after Coomassie stain - (reply: 7)
1116. Western Blot with Plasma - (reply: 7)
1117. Western Transfer and Detection of small protein - (reply: 6)
1118. 2D protein gel for miRNA target screening? - (reply: 2)
1119. Immunoprecipitation Question - Is it Possible to pull down a receptor by probing for its ligand (reply: 1)
1120. protein expression from glycerol stock - (reply: 2)
1121. use of Pierce stripping buffer in WB - (reply: 3)
1122. What should be the pH of the leamlli sample buffer? - (reply: 7)
1123. Streptavidin-biotin peptide pull down for silver stain - (reply: 2)
1124. anti-BRCA1 (ab-1) for WB - (reply: 8)
1125. Short peptide stability - (reply: 4)
1126. low MW bands left on gel during transfer to PVDF - (reply: 1)
1127. confusing shape of survivin protein band - (reply: 3)
1128. Abnormal polymerization of acrylamide when preparing SDS-PAGE - (reply: 9)
1129. Troubleshoot: Western; no bands appear in reduced lanes - (reply: 8)
1130. A basic knowledge for RPHPLC - (reply: 2)
1131. 2nd dimension temperture effects? - (reply: 3)
1132. Expression of an N-terminally tagged golgi protein - (reply: 1)
1133. sepharose bead - (reply: 5)
1134. glycine or tris buffer - (reply: 5)
1135. Reproducibility - (reply: 4)
1136. Native PAGE questions - (reply: 1)
1137. size of tagged proteins - (reply: 3)
1138. SDS PAGE problem - (reply: 5)
1139. Questions about IP - (reply: 2)
1140. How to detect protein kinase from lysate - (reply: 4)

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