121. MASCOT results vs 2D gel image : Which one do you trust? - Protein positive hits in MASCOT do not agree with a spot's position!! (reply: 2)
122. Experience with protein G dynabeads? - (reply: 2)
123. About preparing solution for protein biochemistry - (reply: 2)
124. URGENT: software tools for proteomics - (reply: 1)
125. Why Proteins? - Can't figure proteins out, let's find some help? (reply: 4)
126. Nuclear fraction marker for Western Blots - (reply: 4)
127. Sample volume for reverse phase chromatography - (reply: 2)
128. DMSO for protein stability at -80oC - protein storage (reply: 4)
129. how to find mol wt. of specific protein on net? - (reply: 2)
130. size exclusion.. how to reduce tailing. for proteins.. - sillica matrix tsk columns.. (reply: 2)
131. Size exclusion chromatography for resolution of 18 kd protein and 15 and 10 kd d - columns for the range? (reply: 3)
132. oxidized vs native protein? diffeerence expected on Isoelectric focusing gel? - why? (reply: 2)
133. troubleshoot the protein expression - different protein size but the same construct (reply: 1)
134. native and oxidized protein.. separation on Ion exchange chromatography? - possible? method suggestions? (reply: 2)
135. No bands observed on anti-His western blot - (reply: 11)
136. protein extraction from mammalian cell line - preferred protocol? - (reply: 2)
137. Native & SDS - TOTALLY CONFUSED,kindly help me (reply: 7)
138. Dephosphorylation and IP - (reply: 12)
139. how to find the modification enzyme of a target protein - (reply: 1)
140. Western Blot Blocking - Blocking for 1hr vs overnight (reply: 8)
141. Antifungal Proteins - (reply: 1)
142. Stripping buffer - (reply: 5)
143. TCA precipitation - (reply: 3)
144. Monoclonal vs Polyclonal Abs - (reply: 1)
145. 6XHistaq proteins - (reply: 2)
146. How to lyse mitochondria in order to measue complexIII activity? - (reply: 2)
147. minimum protein conc for immunoprecipitation - what's the minimum needed for results (reply: 1)
148. Precipitate proteins but not 1kDa peptide? - (reply: 3)
149. Can Triton be used for 2D PAGE - Detergent compatibility for 2D PAGE (reply: 1)
150. Protein Quantitation - Methods thats are used for measure the low protein concentation (reply: 9)

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