1561. Overexpression of Viral Protein in mammalian cell line - (reply: 3)
1562. GST-protein purification - help me please! (reply: 2)
1563. questions about native gel - (reply: 3)
1564. Is wetblotting better than semi-dry blotting for quantitative purpose? and why? - (reply: 1)
1565. are there any good system to express functional mammalian protein? - I tried using E.coli, but protein is not functional (reply: 15)
1566. question about "pa quality" - What's that? (reply: 3)
1567. Glycosylation - can I do anything with this? (reply: 3)
1568. AT1 receptor expression and quantification (WB) - need advice (reply: 1)
1569. quanitation of protein extracted with trizol - (reply: 7)
1570. Protein Sorting - The models of Protein sorting (reply: 1)
1571. Macrophages - (reply: 1)
1572. Non reducing Co-IP and 2 Ab that detect light chain only - Co-IP help! (reply: 4)
1573. unspecific binding of protein a sepharose - immunoprecipitation problem (reply: 1)
1574. PMSF in AChE purification :unsure: - (reply: 1)
1575. Immunoprecipitation - Can I boil then freeze? - (reply: 2)
1576. Questions about PAGE - (reply: 3)
1577. NF-kB protein nuclear and cytosol isolation - No band separation in cytosol and nucleus (reply: 7)
1578. How to get rid of DNA contamination in protein samples - (reply: 3)
1579. Native Gel for QDs and Peptides - (reply: 8)
1580. Western blotting - How to interprete (reply: 2)
1581. SDS/UREA PAGE PROTOCOL - (reply: 1)
1582. His-trap HP column? - (reply: 6)
1583. Collagen SDS-PAGE - (reply: 3)
1584. Shift of molecular mass after phosphorylation - How many kDa larger? (reply: 4)
1585. Proteins samples not migrating from stacking into resolving gel - (reply: 6)
1586. Protein TCA Precipitation Resolubilization Question - (reply: 3)
1587. Plotting graph - On Microsoft Excel for protein quantitation (reply: 3)
1588. 2D instead of Immunoprecipitation? - (reply: 3)
1589. Need prototocol for membrane fractionation from mammalian cells - (reply: 1)
1590. Verify the protein spots found by 2D using Western blot - The expression level change are different (reply: 2)

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