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1771. The biology activity of espressed protein - (reply: 3)
1772. how to prepare total cell lysate for Western blot? - (reply: 10)
1773. separation of two negatively charged proteins.... - ion exchage chromatography??? (reply: 2)
1774. Immunoprecipitation - is crosslinking neccesary? - (reply: 7)
1775. how can I increase protein expression level - hurry (reply: 2)
1776. Cyclic peptides multimerize! - How to proceed? (reply: 6)
1777. what is the meaning of de -gas the acrylamide gel mix? - (reply: 6)
1778. Imidazole + enzyme activity - (reply: 6)
1779. SDS-PAGE gel preparation - (reply: 10)
1780. Western band distorted by 'bubbles' - (reply: 8)
1781. western blotting membranes - (reply: 6)
1782. Extinction coefficient - (reply: 2)
1783. Western blot ECL quantification without previous Bradford/Lowry assay... - (reply: 3)
1784. N-ethylmaleimide handling - (reply: 5)
1785. Extra band in western blot - (reply: 1)
1786. concentrating protein from different lysis buffers? - Can you concentrate proteins from differnt buffers and resuspend all in the same (reply: 5)
1787. T-protein reducing faster than its p-form - any idea why?? (reply: 1)
1788. Protein determination - Old Skool style - I'm looking for a decent descriptive protocol for protein detection using th (reply: 6)
1789. ERK by Westerns - (reply: 8)
1790. Protein quantification: BCA with laemmli buffer? - does it work? (reply: 11)
1791. in vitro translation... - why not? (reply: 3)
1792. Allowed gel percentage to seperate proteins of 20kd or less - (reply: 1)
1793. quantification of WB bands- how to get similar values from different experiments - (reply: 4)
1794. how to do western with 500 kD protein? - (reply: 5)
1795. DsRed fusion protein not glowing - It is there by WB though (reply: 3)
1796. OD and protein - calculation (reply: 2)
1797. Blocking Western with Fab fragment - (reply: 3)
1798. Western blot bands fade out - (reply: 9)
1799. stable or transient transfection method? - for protein expression (reply: 4)
1800. Protein didn't express - why? - M15 E. coli culture and pQE30UA vector; pTrcHis2TOPO and TOP10 E. coli cells (reply: 10)
1772. how to prepare total cell lysate for Western blot? - (reply: 10)
1773. separation of two negatively charged proteins.... - ion exchage chromatography??? (reply: 2)
1774. Immunoprecipitation - is crosslinking neccesary? - (reply: 7)
1775. how can I increase protein expression level - hurry (reply: 2)
1776. Cyclic peptides multimerize! - How to proceed? (reply: 6)
1777. what is the meaning of de -gas the acrylamide gel mix? - (reply: 6)
1778. Imidazole + enzyme activity - (reply: 6)
1779. SDS-PAGE gel preparation - (reply: 10)
1780. Western band distorted by 'bubbles' - (reply: 8)
1781. western blotting membranes - (reply: 6)
1782. Extinction coefficient - (reply: 2)
1783. Western blot ECL quantification without previous Bradford/Lowry assay... - (reply: 3)
1784. N-ethylmaleimide handling - (reply: 5)
1785. Extra band in western blot - (reply: 1)
1786. concentrating protein from different lysis buffers? - Can you concentrate proteins from differnt buffers and resuspend all in the same (reply: 5)
1787. T-protein reducing faster than its p-form - any idea why?? (reply: 1)
1788. Protein determination - Old Skool style - I'm looking for a decent descriptive protocol for protein detection using th (reply: 6)
1789. ERK by Westerns - (reply: 8)
1790. Protein quantification: BCA with laemmli buffer? - does it work? (reply: 11)
1791. in vitro translation... - why not? (reply: 3)
1792. Allowed gel percentage to seperate proteins of 20kd or less - (reply: 1)
1793. quantification of WB bands- how to get similar values from different experiments - (reply: 4)
1794. how to do western with 500 kD protein? - (reply: 5)
1795. DsRed fusion protein not glowing - It is there by WB though (reply: 3)
1796. OD and protein - calculation (reply: 2)
1797. Blocking Western with Fab fragment - (reply: 3)
1798. Western blot bands fade out - (reply: 9)
1799. stable or transient transfection method? - for protein expression (reply: 4)
1800. Protein didn't express - why? - M15 E. coli culture and pQE30UA vector; pTrcHis2TOPO and TOP10 E. coli cells (reply: 10)
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