2161. in vitro translation vector - (reply: 3)
2162. Concentrating recombinent antagens - hisTaged antagens purified and neen to concentrated (reply: 1)
2163. Bradford method - filter 575 or 600 instead of 595nm??? (reply: 4)
2164. different colonies produce different amount of protein... - (reply: 6)
2165. concentration of SDS loading buffer ... - 1X buffer seems too little. (reply: 8)
2166. His-tagged antigens - Must the tag be removed?? (reply: 1)
2167. western blotting of tissues - (reply: 1)
2168. Bradford problems with TCA-Ether extracted samples - (reply: 1)
2169. phosphorilation state detection - (reply: 1)
2170. His-tag fusion protein detection problem - (reply: 2)
2171. mRNA or DNA extraction protocol from pichia pastoris - (reply: 5)
2172. DNA OR NUCLEOTIDE CONTAMINATION OF PROTEIN - I NEED TO GET THIS FRAGEMENTED DNA OUT OF MY SMALL PROTEIN (reply: 4)
2173. How often do your proteins have a contamination of endotoxin - (reply: 3)
2174. many bands other than my protein on western... - please help me to explain this... (reply: 5)
2175. Sequential IP's before mass spec analysis - searching for co-ip proteins (reply: 4)
2176. co-ip: how to get rid of the ip-protein? - (reply: 1)
2177. PBS-T vs TBS-T - (reply: 2)
2178. protein precipitation for mouse liver - (reply: 1)
2179. His tag cleavage by Thrombin - (reply: 2)
2180. antibody storage in skim milk - I want to know % of sodium azide (reply: 8)
2181. bee venom peptides, proteins, enzymes - (reply: 1)
2182. ATP-Independent Protein Degradation ? Pls help - (reply: 4)
2183. problem with gel filtration - (reply: 1)
2184. Favorite Western blot transfer apparatus - (reply: 2)
2185. Immobilon PSQ membrane - high background - (reply: 4)
2186. cell lysis? - (reply: 2)
2187. which enzyme should I select? - (reply: 2)
2188. Protein elution question - (reply: 1)
2189. Enzyme activity - Enzyme activity (reply: 5)
2190. how to increase protein expression in E coli? - (reply: 6)

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