2791. strange expression model - (reply: 2)
2792. cloning genes with incompatible restriction sites - Protein expression (reply: 2)
2793. quantify protein on beads? - (reply: 4)
2794. difficult to dissolve protein? - (reply: 1)
2795. "Help" : how to identify of proteins binds to specific DNA - (reply: 3)
2796. lot of contaminating bands, what should i do - (reply: 2)
2797. Concentrating protein in cerebrospinal fluid for Western - (reply: 1)
2798. His Tag purification - specificity problem (reply: 8)
2799. Proposal for strategy to determine 2 protein interactions? - suggest sugges! (reply: 11)
2800. express a big protein in BL21, get an extra band - (reply: 7)
2801. HELP: Western Blot problem - (reply: 3)
2802. Stat5 - (reply: 1)
2803. How many times do you reuse a primary antibody? - (reply: 7)
2804. pestilential of hplc system - hplc problem (reply: 2)
2805. coomassie - (reply: 3)
2806. ammonium sulfate... again - (reply: 1)
2807. western of a big protein - (reply: 2)
2808. SDS-PAGE gel sticking - Gel sticking to the membrane (reply: 3)
2809. Western Blot stripping - quick and easy protocol? - (reply: 2)
2810. Help!! a-tubulin antibody did not work...... - (reply: 1)
2811. Torn PVDF - Western Blotting (reply: 4)
2812. can i autoclave buffers with imidazole for His-tag purification? - (reply: 2)
2813. His-taged protein is in 8M Urea, how to get rid of urea? - Ni-NTA column purified protein in 8M urea buffer (reply: 3)
2814. pET301 maltose binding - Need sequence and map (reply: 1)
2815. Insect cell sonication protocol? - Protein Purification (reply: 1)
2816. Help: western blot lysis buffer - (reply: 2)
2817. amount of protein to load - (reply: 4)
2818. Lysis buffers for extraction of proteins expressed in E.coli - (reply: 5)
2819. SDS-PAGE loading buffer - 1X, 2X, 4X loading buffer? (reply: 2)
2820. Sonication query - How long do people sonicate samples? (reply: 3)

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