2761. Native Gel Staining?? - So I can electrolute proteins by cutting gel slice (reply: 4)
2762. dialysis of purified protein - (reply: 5)
2763. Protein isolation for western - (reply: 8)
2764. Expression even IPTG induction - (reply: 1)
2765. Reliable vendor that sells small aliquots of Antibodies? - (reply: 1)
2766. Random Background Noise for Western Blots - I'm getting noise only in some lanes (reply: 1)
2767. Native non SDS PAGE - (reply: 1)
2768. His-tagged protein co-eluted with associated protein - (reply: 4)
2769. PROBLEM: Western blot of a 220 kd nuclear protein - (reply: 3)
2770. extraction of extracellular protein of C. neoformans - extraction method (reply: 3)
2771. Actin Polymerization Assay...Anyone? - Native or Denatured Protein (reply: 1)
2772. His6 tag quite problematic. - Is that true? (reply: 3)
2773. protein suspended in diff buffer changes mobility - Will differnt salt concentration in buffer interfe (reply: 2)
2774. Too many unspecific bands with B-actin - Western Blot (reply: 5)
2775. Help with western blots please - (reply: 5)
2776. Immunoprecipitation - removing antibody? (reply: 4)
2777. need urgent help for IP - (reply: 3)
2778. Prokaryotic and Mammalian Expression Vectors - Why do they have to be different? (reply: 2)
2779. protein A tagged fusion protein - (reply: 1)
2780. help-ammonium sulfate precipitation - (reply: 1)
2781. aggregates in SDS-PAGE? - double mw of proteins in SDS-page (reply: 2)
2782. Tissue prepatation for WB and other issues - (reply: 5)
2783. Sonicating cell lysate for Westerns - (reply: 2)
2784. Western Blot troubleshooting - (reply: 7)
2785. Temperature for protein expression - (reply: 2)
2786. in-vivo method of identifying 2 protein binding? - (reply: 3)
2787. separation of nucleus protein - lysis buffer (reply: 1)
2788. Li-Cor WB optimisation - WB background at y680 of Li-Cor (reply: 1)
2789. MW of protein on SDS-PAGE different from actual mass - (reply: 4)
2790. Problem with TCA and western blot - (reply: 3)

© 1999-2008 Protocol Online, All rights reserved.