1921. Advice wanted on Western Blotting materials - (reply: 5)
1922. trypsin peptide extraction storage - (reply: 3)
1923. western blot ECL vs fluorescence - (reply: 2)
1924. How would you increase separation on PAGE? - (reply: 16)
1925. Tricine SDS-PAGE gel under non-reducing - please tell me a protocol (reply: 1)
1926. problems in concentration protein solution - (reply: 8)
1927. What is the best assay for protein interaction analysis? - (reply: 1)
1928. Gst tag purification problem - (reply: 5)
1929. Gel permeation & filtration - difference plz? (reply: 1)
1930. Question about proteinase cleavage sites - (reply: 1)
1931. Protease Inhibitors - (reply: 8)
1932. problem with immunoprecipitation - (reply: 1)
1933. is there any problem if the fusion protein was used directly as the antigen? - fusion protein was used directly as the antigen (reply: 4)
1934. what is the molecular weight of fusion protein in pET-32a - molecular weight of the fusion protein in pET-32a (reply: 3)
1935. FLAG M2 affinity gel agarose storage condition - (reply: 1)
1936. what makes a protein insoluble? - (reply: 3)
1937. Pellet after centrifuging - (reply: 2)
1938. Concentration of secreted protein - ...step before IP.... (reply: 1)
1939. Pellet the proteins!? - (reply: 4)
1940. Co-IP experiments, Ratio of amount of cells/lysis volume/ug antibodies - (reply: 2)
1941. Loading control - (reply: 3)
1942. normalizing for loading and transfer error during western blot - (reply: 4)
1943. Protein determination in a insoluble pellet - (reply: 6)
1944. Protein purification wash problem - (reply: 1)
1945. protein storage following purification - (reply: 1)
1946. Expression temprature - (reply: 3)
1947. urea/glycerol PAGE and cutting out the band... - cant find out protocols (reply: 1)
1948. protein elution from PAA gels - (reply: 2)
1949. Immunoaffinity chromatography-help - protocol for coupling of antibodies (reply: 2)
1950. Problem in protein preparation in 2-D electrophoresis - (reply: 1)

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