451. Membrane Protein Extraction and Analysis Questions - (reply: 1)
452. Do you add Triton-X in your lysis buffer? - (reply: 7)
453. Proteomics - Determination of structure of Protein. (reply: 2)
454. Isotype control for Co-ip - do you really use it? (reply: 1)
455. Separation of piperidine from proteins after AIC - (reply: 1)
456. IP and silver stain - how much to start w/? (reply: 1)
457. Transformation protocol for Bacillus subtilis needed - (reply: 1)
458. DNA, RNA, protein acetone precipitation? - (reply: 2)
459. ER membrane prep for 2D gels - liver micrsosomes (reply: 4)
460. Is Co-IP with natural protein a fantasy? - (reply: 6)
461. DTT or mercaptoethanol - (reply: 5)
462. polyclonal antibodies go bad - when stored at -80???? (reply: 5)
463. Protein cocentrators / centrifugal filter tubes - (reply: 3)
464. question about protein molecular weight - (reply: 12)
465. Dynabead magnets - new to this technology (reply: 3)
466. Procols and equipment for isoelectric focussing - (reply: 3)
467. Trouble when extracting Myosin Light Chain for WB - (reply: 1)
468. collagen type I - (reply: 1)
469. How important is Ca2+ to thrombin cleavage? - (reply: 2)
470. Co-IP in sonicated whole cell lysates - (reply: 3)
471. isoform distribtuion and bioactivity? - (reply: 1)
472. Ab's production from recombinant proteins (2) - new idea (reply: 2)
473. mind blogging question - (reply: 5)
474. buffer for anion exchange chromatography of basic proteins - (reply: 1)
475. Ab's production from recombinant proteins - (reply: 4)
476. how to define an appropriate protein reaction buffer? - (reply: 1)
477. Can you share a Blue Native PAGE protocol? - (reply: 2)
478. amount of protein for MALDI-TOF - (reply: 5)
479. Electro Elution of my protein after SDS-PAGE - (reply: 7)
480. Inclusion bodies prep - (reply: 1)

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