1681. Factor Xa Cleavage - MBP-Factor Xa Cleavage (reply: 2)
1682. how to extract nuclear protein - help (reply: 8)
1683. help-SDS-PAGE of heavy proteins - (reply: 7)
1684. Concentrating proteins for N-terminal sequencing - (reply: 2)
1685. Hemin amplifying ECL signal?!? - (reply: 5)
1686. Western Blotting detection Antibodies........... - Are anti-sheep and anti-goat HRP interchangable? (reply: 2)
1687. proteinase K - (reply: 2)
1688. Nuclear Marker for Western Blotting - (reply: 3)
1689. His-tag protein is lost - (reply: 5)
1690. Dsitinct Bands on Ponceau Stain After Sonication? - (reply: 4)
1691. biotinylated proteins purification from free biotin - dialysis vs gel filtration or what else (reply: 10)
1692. Blue Native Gel Electrophoresis for membran protein complex - (reply: 1)
1693. detection of a secreted recombinant protein - (reply: 2)
1694. NI-NTA colunm purification - no bands after purification (reply: 2)
1695. OD variation - with pH (reply: 1)
1696. Plasmid instability - why and how do i check? (reply: 1)
1697. IEF for pI 9.4 protein - ? (reply: 1)
1698. Extinction Co-efficient of a protein - how d i find out? (reply: 3)
1699. SEC for basic protein - column, buffer (reply: 4)
1700. km value and Vmax - (reply: 2)
1701. Fetal Bovine Serum composition - (reply: 6)
1702. Will antibody need any buffer to make link with bead - Which buffer should I use to link bead with antibody (reply: 1)
1703. EMSA - Polyd[i-c] - (reply: 2)
1704. "Ponceau S" distorting the bands in western blot? - (reply: 5)
1705. Bacterial antibodies as the controls for Western - (reply: 1)
1706. Epitope tags - (reply: 1)
1707. sequencing cyclic peptides - (reply: 1)
1708. problem in my native gel - (reply: 4)
1709. protein precipitation during concentration - (reply: 5)
1710. question about an oddity - (reply: 6)

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