541. VERY SLOOOOOW running gels (SDS-PAGE) - (reply: 8)
542. Protein Expression problem - N-End Rule?? (reply: 1)
543. IP first-timer - doubts on equal protein concentration control (reply: 2)
544. Protein will stripped by overwahsing? - (reply: 12)
545. extra bands on SDS PAGE gel - (reply: 4)
546. phosphoprotein - (reply: 1)
547. no protein expression - (reply: 2)
548. Protein Expression Problem - N-end rule?? (reply: 2)
549. Western blot with Adipose cells - (reply: 1)
550. Detecting Transcription factors in western blot - Hard time detecting LEF-1(TFs) (reply: 2)
551. Co- IP of 53 KD proteins - Immunoprecipitation (reply: 2)
552. Protein stability in acetonitrile - storage conditions? - (reply: 1)
553. Sensitive protein assay - (reply: 2)
554. western blots - survivin - detection of survivin in Panc1 cells (reply: 2)
555. Mycobacterial protein extraction... without chemicals! - (reply: 3)
556. Superdex or Sephacryl? - protein purification by size exclusion chromatography (reply: 7)
557. when concentrating my protein sample my solution turns a yellow colour ? why? - (reply: 2)
558. western background problem - (reply: 3)
559. Does trypsin digest integral plasma membrane proteins? - (reply: 2)
560. protein band is shifted after incubation with plant protein extracts! - (reply: 2)
561. protein complex purification taptag - (reply: 2)
562. Schock freezing - cell disruption? - (reply: 3)
563. Organelle separation - buffer, homogenization, centrifugation force? - (reply: 1)
564. Gel loading Question - (reply: 1)
565. Primary-Secondary Antibody Question - (reply: 5)
566. protein precipitation - basic question (reply: 1)
567. Stability of GST-Fusionproteins with Precission-site - (reply: 1)
568. WESTERN BLOT : upper band at 45 kDa in blot of cytochrome c - (reply: 2)
569. Cannot get rid of GST tag - (reply: 6)
570. What is the most effective method of protein precipitation? - (reply: 2)

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