241. Help! questions on DNA pull-down assay - (reply: 7)
242. Urea and Western Blot - (reply: 4)
243. ammonium sulfate precipitation & urea - (reply: 9)
244. WB antibody to be used in IHC - (reply: 7)
245. mouse and rabbit antibodies in western blot - (reply: 2)
246. how to prepare 2d sample after coimmunoprecipitation - (reply: 3)
247. How to dilute an antibody? - (reply: 2)
248. Problem with blastpgp - (reply: 1)
249. Yeast 2 hybrid Colony lift Assay problem - (reply: 4)
250. interested protein shows in every sucrose fraction! help! - (reply: 3)
251. IP elution off of dynabeads - boiling gives a smear (reply: 4)
252. Modification of cysteine in my small peptide? - (reply: 2)
253. Protein extraction supernatant turns black/sooty color - (reply: 4)
254. anti-phosphoAsp - (reply: 1)
255. western blot--protein concetration - too dilute?? (reply: 3)
256. ProteoJuice Protein Transfection - (reply: 2)
257. experimental determination of protein function - (reply: 2)
258. 2-D gel analysis - (reply: 3)
259. Sodium azide - (reply: 1)
260. control sample as a reference for Revers-phase HPLC - quantify and identify by RP-HPLC (reply: 2)
261. How to separate proteins of equal size - (reply: 3)
262. Lysis Buffer for Elisa - (reply: 1)
263. Bradford assay questions - (reply: 19)
264. Use of protease inhibitors when the target protein could be a protease that inte - (reply: 2)
265. Using urea in ion exchange chromatography of proteins - (reply: 1)
266. How to demonstrate protein-protein interaction - How to demonstrate that a protein is a receptor (reply: 10)
267. anti-phosphoSer and anti-phosphoThre antibodies - Which brands / clones are good? (reply: 1)
268. upscaling protein production - is type of vector and host strain crucial factors? (reply: 1)
269. Sense of isoelectric focusing of protein-fraction from ion exchange chromatograp - (reply: 2)
270. Anion exchange column overloading. Peaks in flow through. - (reply: 1)

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