1051. EMSA probe - (reply: 2)
1052. Protein purification - (reply: 3)
1053. Insufficient transfer in Western blotting - (reply: 8)
1054. vertical Isoelectric focusing - cathodic drift and plateau formation of pH gradient (reply: 1)
1055. protein extraction from SDS-PAG (GeBAflex) - your opinion about usefulness (reply: 1)
1056. western blot protocol - (reply: 1)
1057. NaPi buffer - What is NaPi? (reply: 2)
1058. adhesion molecules by westerns - (reply: 3)
1059. coomssie after electroelution - (reply: 4)
1060. how to see low kd protein in vitro transcription/translation? - (reply: 4)
1061. Beta-actin, double bands - Problems with my western blot (reply: 3)
1062. rehydrate dried gel - (reply: 4)
1063. How to the modification of a protein? - (reply: 2)
1064. western with nuclear extract - does high salt/glycerol affect running (reply: 2)
1065. How to determine protein concentration - how to determibne particular protein concentration from a tissue homog (reply: 2)
1066. western blot transfer - what's your opinion? (reply: 6)
1067. GST pulldown- troubleshooting - (reply: 3)
1068. reducing and non-reducing condition in westernblot - (reply: 4)
1069. SDS PAGE band migrates smalle when reduced?! - (reply: 2)
1070. GTP hydrolysis assay - please help me pass my qualifying exam!!! (reply: 1)
1071. Retinoic Acid Receptors Protein Extraction - (reply: 2)
1072. Fix&Perm, biotin-SA protocols, blocking of nonspec. binding - (reply: 4)
1073. Blokcing step of Western blotting - (reply: 5)
1074. Good review about protein expression in E.coli - (reply: 1)
1075. Protein purification - (reply: 3)
1076. GST-Purification problems - (reply: 2)
1077. Protease cutting at KK (lysine-lysine) points - know of any..?? (reply: 1)
1078. SDS-PAGE: unstained marker problem - (reply: 4)
1079. Immunoprecipitation - problem with sepharose beads - (reply: 3)
1080. Quick western method with pvdf? - anyone tried it? (reply: 7)

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