31. What do u think about my protocol for EMSA with antibodies? - EMSA experts out there??? (reply: 1)
32. PMSF precipitate? - (reply: 5)
33. protein concentration measurement - (reply: 4)
34. 2D gelelectrophoresis: prestaining of proteins - (reply: 4)
35. general protein purification from mouse tissue - (reply: 4)
36. SDS-PAGE sample preparation - (reply: 3)
37. Secondary antibody - (reply: 2)
38. Different intensity present in reference protein even though BCA performed - (reply: 1)
39. How long a membrane can be saved stripping? - (reply: 3)
40. sticked protien - (reply: 2)
41. could you comment on this Sample Preparation Protocol for SDS-PAGE from Muscle t - (reply: 4)
42. High background in western blot - (reply: 4)
43. cell fractionation and low protein concentration in cytosol - (reply: 1)
44. Technique to estimate the level of protein expression from media - (reply: 2)
45. protein concentration of samples in Laemmli buffer - (reply: 2)
46. GST pull down assay - negative control stops working (reply: 2)
47. Protein preicipitated after Dialysis - (reply: 7)
48. Methods of concentrating Protein lysate - (reply: 2)
49. WB vs Immunostaining - (reply: 1)
50. How to normalize ELISA measurements? - Total protein, cell number, or DNA? (reply: 1)
51. 1% boiling buffer for protein extractions - (reply: 3)
52. Volatile Buffers for pH 3 - 10 - Generating a list of volatile buffers for mass spectrometry (reply: 1)
53. efficient substitute of the pulse-chase analysis? - (reply: 2)
54. 2D-electrophoresis - “hollow” effect and streaking problems (reply: 9)
55. Protocol for frozen CPDA blood isolation of haemoglobin - Need help! (reply: 1)
56. loading control - (reply: 1)
57. amino acid homology - %identities vs %positive identities (reply: 2)
58. GST-tagged protein expression problem-plz help - (reply: 3)
59. pROTEIN DOMAINS - new to proteomics (reply: 3)
60. Membrane protein problem - Problem in getting signals (reply: 3)

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