2341. what is the appropriate SDS-PAGE concentartion to be used? - (reply: 3)
2342. small sized protein tailing off - (reply: 1)
2343. TCA/DOC precipitation - (reply: 1)
2344. Protein expresses wrong size - (reply: 5)
2345. chitinase activity - very crucial!!!! (reply: 1)
2346. Imidazole removal - want to get rid of Imd (reply: 2)
2347. insoluble His-proteins - (reply: 3)
2348. Expression interference from His-tag - transcription protein (reply: 2)
2349. What is the best nuclear protein marker? - Fractionation (reply: 3)
2350. lysis is not translucent after urea treatment - need help with His tag fusion protein purification (reply: 2)
2351. Tips for Western Blot of Low Molecular Weight Protein - (reply: 4)
2352. Receptor identification - (reply: 3)
2353. Fluorescence - (reply: 4)
2354. Western problem - very challenging - distorted band, non-reproducible, generally awful (reply: 5)
2355. white band on WB - Western blot (reply: 6)
2356. Guys who's done immunoprecipitation- help me! - (reply: 5)
2357. Western Blots - Monoclonal or Polyclonal Antibody...? - Which one to use? (reply: 1)
2358. Harvesting of cells before fractionation - escape of proteins? (reply: 3)
2359. his tag protein not expressing - I am stuck !!! (reply: 6)
2360. bacteria...our small factories - ? (reply: 4)
2361. GST Tag Cleavage - (reply: 2)
2362. 6 His tagged protein purification - (reply: 1)
2363. Phosphorylation of TP53 - (reply: 3)
2364. Isolation of proteins - 2 questions that I get stuck (reply: 2)
2365. PBS buffer + HEPES buffer - can be mixed together? - Can I mix two protein in different buffers, same pH? (reply: 2)
2366. PRIMER design - primer dimers (reply: 1)
2367. Removing salt and immidazole from purified Protein - (reply: 2)
2368. Spotting peptides on PVDF - (reply: 2)
2369. How to analyze antigen-antobody complex on gel? - Which gel will NOT break the complex? (reply: 3)
2370. Handling of cell extracts - (reply: 1)

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