1351. Phoshorylation in SDS Page - Visible shift? (reply: 4)
1352. Need help with WB/IP sensitivity issues - As in way too sensitive! (reply: 3)
1353. why not boiled before electrophoresis? - (reply: 9)
1354. Protein purification before mass-spec - need sample purification methods (reply: 4)
1355. streaking in 2D gels - (reply: 2)
1356. Memrane for Western Blot- which are the best? - (reply: 5)
1357. Anti-digoxigenin Immunoprecipitation of Proteins - (reply: 3)
1358. 1% SDS lysis buffer - (reply: 3)
1359. 2D PAGE problem - (reply: 7)
1360. question on pulldown - (reply: 6)
1361. reversible stain? - (reply: 4)
1362. what these components do in the buffer used in protein purification - (reply: 5)
1363. protein folding- looking for renaturation buffer - (reply: 1)
1364. temperature of silver staining - (reply: 3)
1365. ELISA Ab question - denatured protein and extract - If I extract denatured protein... (reply: 2)
1366. Will the membrane-associated protein aggregate after the removal of detergent? - (reply: 1)
1367. Low protein yield with immunoprecipitation - (reply: 3)
1368. immunoprecipitation question - (reply: 5)
1369. Protein extraction from SDS gel - (reply: 6)
1370. Lysate Sonication - (reply: 3)
1371. Codon optimization, does it matter? - (reply: 3)
1372. Protein loading control for RAW cell supernatants - Western blot loading control (reply: 2)
1373. Can ponceau expire? - (reply: 2)
1374. co-ip - (reply: 3)
1375. Can I just skip RENATURING? - Difficult native prep want to use denaturing (reply: 5)
1376. guanidinehydrochloride vs. guanidineisothiocyanate - Is there a difference concerning ability to denature proteins? (reply: 2)
1377. Protein A or protein G for co-IP? - (reply: 3)
1378. Help: Can't see any positive staining at all... SDS PAGE gel - (reply: 5)
1379. In Vitro Translation --> EMSA - (reply: 1)
1380. SDS-PAGE protein aggregation and precipitation - (reply: 1)

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