2101. Developing Gel instead of PVDF - (reply: 3)
2102. bigger band after reduction with dithithreitol - (reply: 1)
2103. western blot - missing expected band - (reply: 1)
2104. western blot secondary antibody incubation time - will long incubation cause high background? (reply: 3)
2105. MM yeast two hybrid - bradford measuring of yeast proteins - (reply: 3)
2106. Stains for proteins.. - Silver v/s Coomassie (reply: 1)
2107. Problems with cell lysate - (reply: 4)
2108. Help to separate fungal proteins - (reply: 9)
2109. Choice of resin for size exclusion chromatography - (reply: 1)
2110. Multiple unspecifc bands in IP using Protein A sepharose + silver staining - (reply: 2)
2111. Washes for Western, PBS or TBST? - (reply: 12)
2112. protein purification - (reply: 1)
2113. protein concentration - (reply: 6)
2114. Western blot bands look like 'upside down smiles' - (reply: 4)
2115. how to identify proteins on MS? - (reply: 1)
2116. Use the Pichia secreted expression system - White ppt formed in the supernatant after stored at -80 C fridge (reply: 4)
2117. overall strucure change - amino acid mutation (reply: 1)
2118. native gel -- protein migration - (reply: 1)
2119. Problem whith b-actin bands - (reply: 1)
2120. PARP as measurement of apoptosis - (reply: 2)
2121. Low Km value - (reply: 1)
2122. do you sonicate or digest with lysozyme? - which one do you prefer and why.... (reply: 21)
2123. Immunprecipitation of phosphorylated proteins - (reply: 1)
2124. Measure OD600 when perform the protein expression - (reply: 6)
2125. Problem choosing the right kind of Precast gels - (reply: 1)
2126. Methanol in transfer? - (reply: 5)
2127. sds page - (reply: 1)
2128. transfer through the PVDF membrane - (reply: 5)
2129. Problem on MAPK SAPK/JNK detecting - (reply: 10)
2130. Protein is in insoluble fraction during overexpression in E. coli - (reply: 9)

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