2251. problem transfering small proteins - (reply: 4)
2252. Mitochondria isolation - (reply: 2)
2253. denaturating protein electrophoresis - (reply: 5)
2254. any idea about running small size of protein? - (reply: 6)
2255. Expression of N-terminal GFP fusionproteins - Proteins with N-terminal signalpeptide (reply: 3)
2256. Low yield at His tagged-peptide production in labeling media - (reply: 1)
2257. Detecting proteins in Mouse embryonic fibroblast - Any tips on making cell lysates of MEFs? (reply: 2)
2258. NIH3T3 protein expression - it isn't working (reply: 1)
2259. SIZE OF PHOSPHORYLATED PROTEINS - (reply: 6)
2260. Lysis buffer with 1% tritonX - Kindly sugget me its other ingredients (reply: 1)
2261. Magnetic beads - (reply: 1)
2262. reducing and nonreducing buffer, SDS-PAGE - (reply: 3)
2263. Dephosphorylation of protein in tissue lysate - going insane,please help! (reply: 1)
2264. purification of prot interacting with a probe in EMSA - (reply: 8)
2265. pI under denaturing conditions - Plzz reply quick..Tkss! (reply: 4)
2266. Blotting time and voltage for a 20kd protein - (reply: 4)
2267. Western blots - fuzzy below 100 kDa - (reply: 3)
2268. Gel Filtration/Protein Precipitation - (reply: 1)
2269. Type I collagen - extraction and purification - (reply: 1)
2270. DNA in western blot - (reply: 4)
2271. Transfer high molecular weight proteins - (reply: 4)
2272. sodium azide alternative - sodium azide alternative (reply: 1)
2273. How to concentrate protein that already in SDS buffer and measure concentration? - Help please (reply: 4)
2274. Buffer preparation - Preparation of sodium phospate buffers (reply: 2)
2275. Horseraddish-Peroxidase (HRP) "lifetime" - (reply: 5)
2276. CoIP optimization - How to see weak interactions (reply: 4)
2277. extraction of protein from aluminium hydroxide formulated vaccine - extraction of protein (reply: 1)
2278. protein loss in sterile filtration - suggest the 0.22 micron filter (reply: 4)
2279. isolation of protein from blood - isolation of protein from blood (reply: 1)
2280. Problem of protein expressio in different medium - help! (reply: 1)

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