1291. What to load in empty well on SDS gel? - (reply: 8)
1292. Blotting for weekend rather than overnight - What problems could this cause? Will it work? (reply: 2)
1293. western vs elisa - (reply: 4)
1294. phosphorylation of a protein - (reply: 6)
1295. Filter paper assay - have u ever tried?? (reply: 1)
1296. Problem: High background using RIPA buffer with the bradford assay - (reply: 7)
1297. protein precipitation upon dialysis - (reply: 5)
1298. I miss two Proteins from my overexpression in BL21 DE3 - (reply: 1)
1299. Cytochrome c as well as caspase 3 (or other caspases)? - (reply: 3)
1300. ugly bands on sds-page of high mw protein (mTOR) - (reply: 9)
1301. what speed to centrifuge down agarose bead? - (reply: 4)
1302. Sudden, Non specific pulldown in Co-IP's - Why? (reply: 1)
1303. Lots of bands on immunoblot for an expressed protein - (reply: 8)
1304. Ghost bands in Western Blots - (reply: 5)
1305. Mild, non SDS based elution conditions for disrupting protein-protein interactio - (reply: 2)
1306. faster proteins - (reply: 1)
1307. Myc tag and endogenous myc - Is there endogenous myc expressed in some fibreblast cells? (reply: 3)
1308. protein separation - (reply: 7)
1309. Big protein - (reply: 6)
1310. proteomic study at 1-D and 2-D electrophoresis. - wants your opinion ! (reply: 5)
1311. Purification protein from inclusion bodies - (reply: 4)
1312. size of PCR product in PAGE - (reply: 5)
1313. questions about secondary antibody - (reply: 2)
1314. protein bands are not migrating over to the whole gels. - please suggest me something, very urgent ! (reply: 8)
1315. Elcetrophoresis Question ( Protein Size) - (reply: 5)
1316. in vitro translation - how is the protein purified? (reply: 12)
1317. Immunoprecipitation - (reply: 2)
1318. Quantity One for bands - (reply: 5)
1319. yeast two hybrid vectors - which one r u using? (reply: 2)
1320. Ion exchange chromatography question - (reply: 6)

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