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2881. Benzamidine Bead Problem - (reply: 1)
2882. Reducing agent in Ni column - Ni-chelated resin turn black under 2-mercap (reply: 4)
2883. smear of loading buffer observed - SDS-PAGE gel (reply: 5)
2884. protein quantitation - for equal western loading (reply: 2)
2885. there seems no induction of my recombinant protein - (reply: 4)
2886. How to load 60 micro-litre sample to a single well in SDS-PAGE? - SDS-PAGE (reply: 8)
2887. Protein Homogenization - Soluble and insoluble protein (reply: 3)
2888. detection of protein phosphorylation using M.S. - (reply: 4)
2889. Protein produced with brown color? - I have some problem in protein expression (reply: 1)
2890. can I freeze the gel slice for protein sequencing - (reply: 1)
2891. His-Tag purification using Qiagen Ni-NTA spin column - Extra band of unknown protein beside target protei (reply: 2)
2892. question about the protein extraction - (reply: 1)
2893. recombinant protein lost immunological activity - (reply: 1)
2894. Storing a protein membrane overnight? - (reply: 5)
2895. total stat5 levels in mammary gland - are they always the same? (reply: 1)
2896. How to identify a protein that binds a defined DNA sequence - (reply: 4)
2897. sensitivity and staining specificity of sypro vs. proQ - (reply: 1)
2898. Utility of the K2HPO4 - (reply: 2)
2899. p-Smad2 problems in western blot - TGF-beta and p-Smad 2 (reply: 1)
2900. ABC technique with Western blot - Do you get extra band with ABC? (reply: 1)
2901. Trouble with Western Blot Transfer (Poor transfer) - Could the protein isolation or homogenization be the cause? (reply: 4)
2902. GST tag - expression with GST (reply: 11)
2903. electroblotting - protein transfer (reply: 1)
2904. Ni-NTA problem - His-tag protein that never bound with Ni-NTA agarose (reply: 10)
2905. His-tag protein purification - Question (reply: 9)
2906. Reuse antibodies for further incubation in Western blot - (reply: 5)
2907. coomassie blue stained gel - possible to obtain good blotting onto membrane? (reply: 3)
2908. Protein with His6 tag - (reply: 2)
2909. Need protocol for SDS-Page on protein extracted from soil - (reply: 1)
2910. Nuclear protein extract (Scrape or Trypsinize)? - (reply: 1)
2882. Reducing agent in Ni column - Ni-chelated resin turn black under 2-mercap (reply: 4)
2883. smear of loading buffer observed - SDS-PAGE gel (reply: 5)
2884. protein quantitation - for equal western loading (reply: 2)
2885. there seems no induction of my recombinant protein - (reply: 4)
2886. How to load 60 micro-litre sample to a single well in SDS-PAGE? - SDS-PAGE (reply: 8)
2887. Protein Homogenization - Soluble and insoluble protein (reply: 3)
2888. detection of protein phosphorylation using M.S. - (reply: 4)
2889. Protein produced with brown color? - I have some problem in protein expression (reply: 1)
2890. can I freeze the gel slice for protein sequencing - (reply: 1)
2891. His-Tag purification using Qiagen Ni-NTA spin column - Extra band of unknown protein beside target protei (reply: 2)
2892. question about the protein extraction - (reply: 1)
2893. recombinant protein lost immunological activity - (reply: 1)
2894. Storing a protein membrane overnight? - (reply: 5)
2895. total stat5 levels in mammary gland - are they always the same? (reply: 1)
2896. How to identify a protein that binds a defined DNA sequence - (reply: 4)
2897. sensitivity and staining specificity of sypro vs. proQ - (reply: 1)
2898. Utility of the K2HPO4 - (reply: 2)
2899. p-Smad2 problems in western blot - TGF-beta and p-Smad 2 (reply: 1)
2900. ABC technique with Western blot - Do you get extra band with ABC? (reply: 1)
2901. Trouble with Western Blot Transfer (Poor transfer) - Could the protein isolation or homogenization be the cause? (reply: 4)
2902. GST tag - expression with GST (reply: 11)
2903. electroblotting - protein transfer (reply: 1)
2904. Ni-NTA problem - His-tag protein that never bound with Ni-NTA agarose (reply: 10)
2905. His-tag protein purification - Question (reply: 9)
2906. Reuse antibodies for further incubation in Western blot - (reply: 5)
2907. coomassie blue stained gel - possible to obtain good blotting onto membrane? (reply: 3)
2908. Protein with His6 tag - (reply: 2)
2909. Need protocol for SDS-Page on protein extracted from soil - (reply: 1)
2910. Nuclear protein extract (Scrape or Trypsinize)? - (reply: 1)
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