1621. Is DTT better then B-mercapto - (reply: 8)
1622. One or two bands with different protein extraction buffer - (reply: 3)
1623. Western blotting signal problem - very weird and does not make any sense. (reply: 10)
1624. Coomassie Blue protein assay - Interference from phenolic acids (reply: 4)
1625. SDS-PAGE over heating and will western work from that gel? - sds page running and western blot of gel run under too much heating (reply: 9)
1626. Membrane protein extraction - What about MEM-PER kit (Pierce)? (reply: 1)
1627. EMSA Supershift for STAT3 - (reply: 15)
1628. Pierce BCA analysis problems :/ - (reply: 3)
1629. Protein Structure - (reply: 4)
1630. Protein forced aggregation - conditions? (reply: 1)
1631. Protein refolding from Inclusion bodies - how to do and confirm the folding? (reply: 4)
1632. A native PAGE - for a protein, 15.3 kDa, gel/run conditions? (reply: 2)
1633. Protein characterization - what kinda stuff do u do? (reply: 1)
1634. Silver stainging after coomassie? - SDS-PAGE (reply: 5)
1635. Help with Ni-NTA purification of EPSP Synthase protein - EPSP not binding to Nickel beads (reply: 5)
1636. Transcritpion factor and DNA binding - I would like to know!!!! (reply: 5)
1637. A question about supershift assay - Band disappeared but no supershift band (reply: 5)
1638. DNA binding proteins - help (reply: 2)
1639. differentiating differently folded structures of the same protein - what methods? reply asap plz (reply: 4)
1640. stablization in a detergent - (reply: 1)
1641. Elution from immunoprecipitation suitable for 2D-GE - Elution from immunoprecipitation suitable for 2D gel electrophoresis (reply: 1)
1642. Overexpression of 630 aa protein - (reply: 3)
1643. TCA/Acetone precipitation - (reply: 3)
1644. can i still see the GFP reaction after the distruction of cell. - (reply: 1)
1645. Membrane protein extraction - Qiagen extraction kit? (reply: 2)
1646. IP from frozen protein extracts - (reply: 10)
1647. Acrylamide gels: sample preparation with high salt concentration - (reply: 1)
1648. membrane protein aggregation - (reply: 2)
1649. Can we freeze mitochondria after isolation? - (reply: 3)
1650. His tag purification - (reply: 1)

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