2311. problem purifying a his-tag protein - (reply: 2)
2312. need ideas for protein protein interaction - protein interaction assays (reply: 3)
2313. Unexplained His-tag cleavage - (reply: 2)
2314. FLuorescence methods - (reply: 2)
2315. to prepare westen blot transfer buffer, instead of methanol can i use ethanol. - (reply: 3)
2316. Need SDS purple loading sample dye recipe! - (reply: 3)
2317. Condition for sonication of protein solution to get rid of DNA? - How long to sonicate and with what amplitude? (reply: 4)
2318. Who have used beads covered with protein in ELISA? - Are they heavy enought to be washed out? (reply: 2)
2319. Proteases - need papers (reply: 1)
2320. How much concentration of PEG is useful for protein-protein interaction? - (reply: 1)
2321. How to remove LPS from protein solution - (reply: 2)
2322. protein synthesis - (reply: 2)
2323. Urea gel - preparation of urea gel (reply: 2)
2324. How to conjugate the antibody to the beads. - (reply: 4)
2325. Gelatin zymography, problem with running - (reply: 3)
2326. tricin gel problem - shrinking of stacking gel (reply: 6)
2327. Storing SDS-page gels - (reply: 1)
2328. MDR western - (reply: 1)
2329. Different trend of results between silver staining and coomassie blue staining? - (reply: 9)
2330. DH5 Alpha protein expression - (reply: 1)
2331. Extract hemoglobin from blood for starch gel electrophoresis - (reply: 1)
2332. GST protein purification - only 3% bind to beads (reply: 6)
2333. Contamination after purification - (reply: 1)
2334. Proteins expression-temperature inducible vs IPTG inducible system - (reply: 2)
2335. Best electrophoresis set up? Biorad vs. Hoeffer - (reply: 4)
2336. protein can be found by IHC but not WB - (reply: 2)
2337. Qn on affinity column chromatography - regarding some of the mechanism behind it (reply: 1)
2338. why get rid of imidazole in his tag protein purification - (reply: 3)
2339. beta glycerophosphate - where to buy (reply: 1)
2340. IP lysis buffer formulation : why adding a non-ionic detergent if a ? - (reply: 4)

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