2521. protein-protein crosslinking - (reply: 3)
2522. IP and 2D gel analysis - buffer composition (reply: 1)
2523. 2D electrophoresis - (reply: 1)
2524. PVDF staining problem... - (reply: 3)
2525. protein purification - (reply: 1)
2526. protein free medium - (reply: 1)
2527. Antibody testing - what if antigen is not available in a soluble form? (reply: 2)
2528. How to run SDS-PAGE and Western Blotting for small peptide? - Tricks for polypeptides less than 10KD??? (reply: 5)
2529. Harvesting cells for Western - what protease inhibitors do you add to PBS? - (reply: 4)
2530. overexpression problems - (reply: 12)
2531. Expressing GST-Fusion protein - Are larger protein inserts harder to express? (reply: 4)
2532. protein/enzyme activity - lost activity after his tagging (reply: 1)
2533. pH and precipitation - (reply: 2)
2534. IP not working with Trueblot - (reply: 5)
2535. Protein samples still in liquid form at -20C - Storage condition for proteins (reply: 8)
2536. Large scale protein purification - Yellow and cyan fluorescent protein (reply: 4)
2537. Western background - (reply: 2)
2538. loading buffer concentration - (reply: 2)
2539. protein purification - (reply: 3)
2540. peptide expression - (reply: 3)
2541. optomizing IP results - what works for you? (reply: 1)
2542. Staining SDS-PAGE prior to transfer - (reply: 1)
2543. Does imidazole absorbe at 280 nm? - How do you measure your protein content? (reply: 4)
2544. chemiluminescent substrate - western blotting (reply: 2)
2545. Western background - vertical stripes ! urgent - (reply: 3)
2546. How to make western results clean??? - (reply: 3)
2547. background in western - very strange stripes ?! - (reply: 2)
2548. How much 3D structure data is available to date? - (reply: 1)
2549. Troubleshooting flag-tagged secreting protein expression - Troubleshooting, Improvement, tricks! (reply: 5)
2550. Extremely lunatic competent cell - transformation (reply: 11)

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