3031. Electroblotting of Proteins on - transfer buffer without Tris and Glycine (reply: 1)
3032. Fusion In Frame....? - (reply: 2)
3033. "disappeared" Proteins Using Bradford - Bradford reaction does not give correct results (reply: 2)
3034. problems with loss of purified protein due to precipitation - protein purification (reply: 4)
3035. active genes and expression problems! - (reply: 3)
3036. proteomics-HELP! - (reply: 1)
3037. PVDF - western protocl with PVDF (reply: 1)
3038. Troubleshooting sds-page for high mw protein - SDS-PAGE (reply: 2)
3039. problem with spectrophotometer - (reply: 8)
3040. Western Blot - signal doesn't show up on film - (reply: 8)
3041. Western Blots - (reply: 1)
3042. problem on membrane preparation - (reply: 1)
3043. Expression of Proteins for western blot - (reply: 1)
3044. Phosphorylation of protein - Trouble with backphospho (reply: 3)
3045. Western - X-reaction with molecular weight marker (all of a - (reply: 1)
3046. Phosphorylation site and Src - question about a phosphrylation site and an SH3 (reply: 1)
3047. my bands spread as a line trough the gel - (reply: 3)
3048. protein lysates preparation - (reply: 4)
3049. SDS gel troubleshoot - My proteins do not show up in the gel. (reply: 1)
3050. Protein expression troubleshooting - (reply: 2)
3051. help: Problem encountered using GST fusion system - (reply: 3)
3052. EMSA problem - nothing binds (reply: 3)
3053. Problem about gene leakage in P.pastoris - (reply: 1)
3054. his tag removal - (reply: 6)
3055. DNA:Protien Site-Specific Binding Determination - Requesting Information on the Subject (reply: 1)
3056. Major problem with Western blotting - (reply: 2)
3057. Glycoproteins on SDS-PAGE - (reply: 3)
3058. protein expression - How to express protein of a high molecul (reply: 4)
3059. urease purification - urease (reply: 1)
3060. [35s] Met labeling protein protocol ! - (reply: 1)

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