1231. silver stain can even get band with the loading buffer control - (reply: 4)
1232. Western blotting with room temperature buffers - protocol says NOT to use cold buffers (reply: 6)
1233. western to detect ATM - (reply: 3)
1234. Technical question about western blot - (reply: 8)
1235. Western Blot Feedback - (reply: 1)
1236. Trouble with Western blot transfer buffer - (reply: 11)
1237. Dose anyone know IP buffer recipe? - (reply: 6)
1238. 3X flag peptide precipitate out - (reply: 1)
1239. Is PH8 good for flag tag immunoprecipitation? - (reply: 2)
1240. Unreliable Protein Induction - (reply: 1)
1241. white bands in wb - (reply: 4)
1242. ha-tag purification , lots of bands - (reply: 4)
1243. DTT in lämli buffer - (reply: 3)
1244. Frowns in polyacrylamide gel - (reply: 8)
1245. lysozyme - help (reply: 1)
1246. 2 questions in 1....about transferring large proteins...and blotting - (reply: 7)
1247. Overexpressing bacterial protein in mammalian cells - which vector to use? - overexpression in mammalian cells (reply: 9)
1248. western questions - (reply: 3)
1249. typical cell lysate protein concentration - (reply: 6)
1250. Many negative bands in WB - (reply: 4)
1251. Pull down assay---- to study which proteins interact with the "bait" - Which technique is good? (reply: 3)
1252. Questions about protein gel loading and immunoprecipitation - (reply: 4)
1253. Flag-tag purifaction elute w/ peptide or SDS loading buffer - (reply: 1)
1254. wash buffer vs lysis buffer PH 7.4 or 8.0 - (reply: 1)
1255. program to quantitate 2D spots from NCBI - (reply: 10)
1256. Antibody question - (reply: 5)
1257. Formaldehyde Cross-Linking for protein - (reply: 1)
1258. why quick freeze-thaw cycles can improve protein recovery? - (reply: 3)
1259. immunoprecipitate O/N or just 2-3 hrs - (reply: 2)
1260. How to get rid of nuclease in protein preparation? - (reply: 5)

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