Antibody storage - (Aug/04/2007 )
Hi,
I have a couple new antibodies that are recommended for storage at 4 degrees for up to 12 months, I am tempted to store an aliquot in glycerol at -80 to keep it longer. This should be okay right?
Thanks!
-Vicky.ac-
We keep an antibody aliquot in 4C or -20C and the rest of them stay in -80C.
-scolix-
QUOTE (scolix @ Aug 4 2007, 05:23 PM)
We keep an antibody aliquot in 4C or -20C and the rest of them stay in -80C.
Well the company recommended only keeping it a 4 degrees. Usually when we get antibodies in our lab, they are recommended to be stored at -80 with an aliquot at 4 or -20 degrees for everyday use. These say only to store it at 4 degrees, not -80 or -20...I don't really see why. So even in this case? Maybe I should just contact the company?
Thanks!
-Vicky.ac-
There is a pinned topic "Antibodies life" under this same forum. Perhaps you can have a look on that..
-money-
QUOTE (Vicky.ac @ Aug 5 2007, 12:15 AM)
Well the company recommended only keeping it a 4 degrees. Usually when we get antibodies in our lab, they are recommended to be stored at -80 with an aliquot at 4 or -20 degrees for everyday use. These say only to store it at 4 degrees, not -80 or -20...I don't really see why. So even in this case? Maybe I should just contact the company?
Thanks!
Thanks!
is the antibody congjugated with an enzyme (secondary ab)? if so, then it may be to protect the enzymatic activity. our secondaries all say to store at 4C.
-mdfenko-