211. Vertical streaks on WB following IP - (reply: 3)
212. protein degredation - (reply: 3)
213. Fibrinogen quantification in plasma - (reply: 2)
214. X-Gluc storage - (reply: 1)
215. cannot IP anymore - used to be able to (reply: 5)
216. Micro-BCA Assay - (reply: 2)
217. Reverse phase chromatography - protein sample preparation and loading - (reply: 1)
218. FLAG IP incubation time - Duration for FLAG IP (reply: 2)
219. protein absorption at 260nm - (reply: 7)
220. Nuclear extracts from myeloid cells? - (reply: 9)
221. Sample (protein) recovery on 2D gel - (reply: 1)
222. method to separate and quantify plasma proteins - please help! (reply: 10)
223. Polyclonal antibody service - Who can you recommend? (reply: 2)
224. how to prove chaperone activity - (reply: 2)
225. digitonin lysis buffer - (reply: 1)
226. unstable protein expression,pleeeeeese help - (reply: 6)
227. non-specific bands in western blotting - (reply: 3)
228. Large bands (207kDa) showing up in CoIP western - (reply: 1)
229. Tween 20 and nitrocellulose - (reply: 3)
230. how to avoid the keratin contamination form 2-DE gel - kindly help me!!!!!! (reply: 4)
231. IEF: Got stuck with my riddle - Problems with evaluation of my IEF blot (reply: 2)
232. integrin beta1 western - (reply: 1)
233. serum and Akt phosphorylation - Do I have use serum-free media to check pAkt when western blotting? (reply: 4)
234. protein estimation colorimetry.. preferred range.. - statistically more precise & accurate? (reply: 2)
235. Recombinant protein stability - stability issue (reply: 1)
236. How dilute an antibody 1:150000 - (reply: 2)
237. Thrombin digestion for GST - ppt problem (reply: 3)
238. Surprise!Does anyone have this kind of result when doing co-IP? - (reply: 3)
239. RPC column pore size versus protein size - (reply: 1)
240. Influence of size of RPC column on resolution, binding capacity - (reply: 1)

© 1999-2008 Protocol Online, All rights reserved.