571. The best way to confirm an protein-protein interaction? - Between a protein and an intergrator (reply: 4)
572. protein precipitating - (reply: 8)
573. GST fusion protein expression problem - (reply: 1)
574. Dialysis doubt - (reply: 1)
575. [western blotting] Loading control in 6% gel? - Please suggest good loading control protein of high molecular weight (reply: 2)
576. SDS-PAGE Loading Buffer with Ficoll? - (reply: 6)
577. Transfer problem - why the size of SDS-PAGE become too small during the transfer (reply: 7)
578. Protein-A HRP to reduce background from HC/LC - (reply: 1)
579. Expression problem in E.coli system using pETT22b+ from Novagen - low level of expression with unknown reason (reply: 4)
580. WB trouble - no signal of low kd protein - (reply: 5)
581. buffer exchange problem - (reply: 3)
582. Low weight protein - (reply: 5)
583. Coomassie: brilliant blue G or R? - (reply: 4)
584. Alternatives to Bradford - Bradford disturbed by Lysis Buffer (reply: 3)
585. what's the difference between the purification of GST-fused protein and His- - (reply: 4)
586. Is there any good coomassie stain which is comparable to silver stain? - searching for a new staining method for 2D gels (reply: 6)
587. Confirming IP with WB - Can I use the same antibody for both IP and WB? (reply: 5)
588. Subcellular fractionation by centrifugation - Can u tell me a easy protocol? (reply: 7)
589. How to choose the ready gel for western blotting - (reply: 3)
590. immunoprecipitation problem - (reply: 6)
591. Special procedures for phosphorylated proteins? - During isolation and western blot (reply: 3)
592. why my RPHPLC keeps making air bubbles... - (reply: 3)
593. 2x SDS-Loading Lysis buffer: use 2x or 1x? - (reply: 4)
594. Coimmuprocipitation problems - (reply: 6)
595. difference between C18 packing materials? - (reply: 2)
596. blinking results in western blot - (reply: 2)
597. identification of IRES - (reply: 2)
598. Western Blot of cells in Laemmil - (reply: 5)
599. immunoaffinity chromatography-choose of matrix and functional group? - (reply: 3)
600. purpose of sucrose in buffer - organelle separation - (reply: 2)

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