841. FPLC fractionation of lipoproteins (to identify the amounts of VLDL, HDL in seru - FPLC fractionation of serum (reply: 1)
842. Protein quantification on spinal cord lysates - reproducibility issues (reply: 1)
843. Transparent lines in Western Blotting - (reply: 1)
844. Estimating Protein Mass - Best way to quantitiate protein (reply: 1)
845. Transfer during western blotting - (reply: 8)
846. antibodies life - 4oC, -20oC or -80oC (reply: 26)
847. Special Western Blot Troubles - help neede (reply: 8)
848. ELISA antibody-use for WB? - (reply: 6)
849. Smear in WB - (reply: 4)
850. Bath Sonicator vs. Probe Sonicator? - (reply: 4)
851. Zymography/Detecting pro-Zymogen - How does pro-enzyme digest the substrate? (reply: 2)
852. gst purification, got extra band - (reply: 4)
853. protocol for gradient SDS-PAGE - (reply: 1)
854. protein dilution - (reply: 3)
855. problems with phosphate buffer in hydroxyl apatite chromatography - (reply: 1)
856. Western blot: Circular bands - artefact? - (reply: 4)
857. Best co-immunoprecipitation tool to buy? - Where do you buy your beads from? (reply: 4)
858. western blotting - (reply: 1)
859. Co-immunoprecipitation? - (reply: 1)
860. Protease Cocktail Inhibitor Complete from Roche? - (reply: 4)
861. Proteinase K and SDS? - (reply: 2)
862. protocal for membrane extraction/fractionation - (reply: 3)
863. Need to increase protein precipitation yield w/ TCA precipitation protocol - (reply: 3)
864. IP for beginners? - (reply: 4)
865. Surface proteins isolated by biotinylation are contaminated.. - (reply: 3)
866. PMSF? - (reply: 5)
867. what nuclei look like after high salt nuclear extraction - (reply: 11)
868. WB for secreted protein? - (reply: 5)
869. membrane protein western blot - (reply: 3)
870. Protein Crosslinking - Tryin' to Crosslink 2 proteins using -SH group (reply: 4)

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