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1021. Is imidazole bad for gelfiltration columns? - Imidazole on gelfiltration columns (reply: 1)
1022. Trouble with ugly bands in EMSA - (reply: 1)
1023. Negative signals on Immunoblot - how to discuss? (reply: 1)
1024. Silver staining of crystals - To prove it's a complex between 2 proteins (reply: 1)
1025. His tag purification - competition with imidazole or histidine? (reply: 5)
1026. serine protease - (reply: 1)
1027. subcellular fractionation confirmed by western not consistent with microscopy - nuclear protein by microscopy not nuclear in fractionated sample -WHY? (reply: 3)
1028. Polyclonal ABs for IP? - (reply: 3)
1029. Transfer optimization of membrane proteins to PVDF - (reply: 3)
1030. IPTG protein expression in BL21 (or orthers) - how to measure the expression (reply: 1)
1031. crystallisation explanation - (reply: 1)
1032. protein precipitated - (reply: 2)
1033. how much should the shift move above in case of supershift - (reply: 1)
1034. can u guess the molecular weight of a raised antibody? - (reply: 3)
1035. Counting cell numbers - (reply: 6)
1036. IsoThermal Calorimetry - (reply: 1)
1037. Does Ponceau stain of NC membrane interfere with Ab binding? - (reply: 4)
1038. White precipitate after unfreezing from -20 degrees centigrade - (reply: 4)
1039. Bands in IEF gel - How can I extract them? (reply: 3)
1040. silver nitrate high background - (reply: 1)
1041. PVDF membrane - Please help me (reply: 3)
1042. How to purify protein secreted in medium - (reply: 2)
1043. Dendritic cell line media - Dendritic cell line media (reply: 1)
1044. buffers - PBS-T versus TBS-T (reply: 8)
1045. Nature of total proteins can influence the migration? - (reply: 6)
1046. Protease inhibitors - (reply: 8)
1047. finiding the best promoter for induction od toxic protein - protein expression (reply: 1)
1048. Phage Display: Not getting antibodies after 3 rounds of panning - (reply: 6)
1049. Duration of Maximum Formalin fixing for tissue - Formalin fixing for tissue (reply: 1)
1050. same proteins, same antibodies, different results - (reply: 1)
1022. Trouble with ugly bands in EMSA - (reply: 1)
1023. Negative signals on Immunoblot - how to discuss? (reply: 1)
1024. Silver staining of crystals - To prove it's a complex between 2 proteins (reply: 1)
1025. His tag purification - competition with imidazole or histidine? (reply: 5)
1026. serine protease - (reply: 1)
1027. subcellular fractionation confirmed by western not consistent with microscopy - nuclear protein by microscopy not nuclear in fractionated sample -WHY? (reply: 3)
1028. Polyclonal ABs for IP? - (reply: 3)
1029. Transfer optimization of membrane proteins to PVDF - (reply: 3)
1030. IPTG protein expression in BL21 (or orthers) - how to measure the expression (reply: 1)
1031. crystallisation explanation - (reply: 1)
1032. protein precipitated - (reply: 2)
1033. how much should the shift move above in case of supershift - (reply: 1)
1034. can u guess the molecular weight of a raised antibody? - (reply: 3)
1035. Counting cell numbers - (reply: 6)
1036. IsoThermal Calorimetry - (reply: 1)
1037. Does Ponceau stain of NC membrane interfere with Ab binding? - (reply: 4)
1038. White precipitate after unfreezing from -20 degrees centigrade - (reply: 4)
1039. Bands in IEF gel - How can I extract them? (reply: 3)
1040. silver nitrate high background - (reply: 1)
1041. PVDF membrane - Please help me (reply: 3)
1042. How to purify protein secreted in medium - (reply: 2)
1043. Dendritic cell line media - Dendritic cell line media (reply: 1)
1044. buffers - PBS-T versus TBS-T (reply: 8)
1045. Nature of total proteins can influence the migration? - (reply: 6)
1046. Protease inhibitors - (reply: 8)
1047. finiding the best promoter for induction od toxic protein - protein expression (reply: 1)
1048. Phage Display: Not getting antibodies after 3 rounds of panning - (reply: 6)
1049. Duration of Maximum Formalin fixing for tissue - Formalin fixing for tissue (reply: 1)
1050. same proteins, same antibodies, different results - (reply: 1)
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