2461. Biotinylation - (reply: 2)
2462. Aggregation of protein after purification? - (reply: 2)
2463. nuclear extract - protocol for cell fractioning (reply: 1)
2464. LC-MS contamination - (reply: 3)
2465. protein isolation of plant tissue -trizol regent - (reply: 3)
2466. GST fusion protein extraction - (reply: 5)
2467. ask for help on ubiquitination assay in vivo - (reply: 3)
2468. circular dichroism analysis for peptide-protein interactions - (reply: 1)
2469. how to denature protein without SDS and precipitating - PNGase A digestion help!!! urgent! (reply: 1)
2470. Protein analysis of conditioned media - How to prepare media before SDS-PAGE? (reply: 4)
2471. how many times do you re-use these two buffers? - (reply: 5)
2472. why can't I detect my potein? - (reply: 6)
2473. GST beads purification - Need help ASAP (reply: 4)
2474. No transfer of protein on PVDF membrane - Western blot troubleshooting (reply: 5)
2475. Phosphorylation - (reply: 8)
2476. Formaldehyde crosslinking - Compatibility with Mass spec (reply: 2)
2477. Non-radioactive labelling for EMSA? - (reply: 4)
2478. Where did the protein go? - Low protein reading in WB lysates (reply: 4)
2479. Mass Spectrometry - (reply: 1)
2480. disulfide bonds in proteins - (reply: 4)
2481. Help! I am in need of informatiom/a way to obtain a non-fluorescing, color p - (reply: 1)
2482. codon bias? - protein expression (reply: 11)
2483. Serum Preperation for 2D - (reply: 1)
2484. Silly question on His-tagged protein - (reply: 2)
2485. VERY STUPID QUESTION - (reply: 5)
2486. Sonicating vs. homgenizing - (reply: 5)
2487. Streaks appearing between lanes in my western blot - (reply: 3)
2488. Is actin an internal control for nuclear fraction? - (reply: 3)
2489. Appearence of non specific[non 6His tagged] proteins with Ni-NTA purification. - (reply: 16)
2490. Amidoblack staining - (reply: 1)

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