1261. SUMO fusion proteins - problems with SUMO Protease (reply: 1)
1262. problem in immunoprecipitation - (reply: 8)
1263. What to load in empty well on SDS gel? - (reply: 8)
1264. Blotting for weekend rather than overnight - What problems could this cause? Will it work? (reply: 2)
1265. western vs elisa - (reply: 4)
1266. phosphorylation of a protein - (reply: 6)
1267. Filter paper assay - have u ever tried?? (reply: 1)
1268. Problem: High background using RIPA buffer with the bradford assay - (reply: 7)
1269. protein precipitation upon dialysis - (reply: 5)
1270. I miss two Proteins from my overexpression in BL21 DE3 - (reply: 1)
1271. Cytochrome c as well as caspase 3 (or other caspases)? - (reply: 3)
1272. ugly bands on sds-page of high mw protein (mTOR) - (reply: 9)
1273. what speed to centrifuge down agarose bead? - (reply: 4)
1274. Sudden, Non specific pulldown in Co-IP's - Why? (reply: 1)
1275. Lots of bands on immunoblot for an expressed protein - (reply: 8)
1276. Ghost bands in Western Blots - (reply: 5)
1277. Mild, non SDS based elution conditions for disrupting protein-protein interactio - (reply: 2)
1278. faster proteins - (reply: 1)
1279. Myc tag and endogenous myc - Is there endogenous myc expressed in some fibreblast cells? (reply: 3)
1280. protein separation - (reply: 7)
1281. Big protein - (reply: 6)
1282. proteomic study at 1-D and 2-D electrophoresis. - wants your opinion ! (reply: 5)
1283. Purification protein from inclusion bodies - (reply: 4)
1284. size of PCR product in PAGE - (reply: 5)
1285. questions about secondary antibody - (reply: 2)
1286. protein bands are not migrating over to the whole gels. - please suggest me something, very urgent ! (reply: 8)
1287. Elcetrophoresis Question ( Protein Size) - (reply: 5)
1288. in vitro translation - how is the protein purified? (reply: 12)
1289. Immunoprecipitation - (reply: 2)
1290. Quantity One for bands - (reply: 5)

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