1981. Stabilization of protein without glycerol - protein has to be ip injected in mice (reply: 1)
1982. Reason for Totally Black Blot - (reply: 20)
1983. non-specific binding to anti-Flag beads - (reply: 4)
1984. Cell lysing for western blot (Help!) - (reply: 2)
1985. Ramdom competitor in Gel Shift Assay - EMSA expert, please help! (reply: 1)
1986. 2-D electrophoresis problem... strange spots - (reply: 2)
1987. pHing Tris - (reply: 2)
1988. Western Blotting substrate - Lumi-Light Western Blotting Substrate (reply: 4)
1989. special band in BL21(DE3) - protein expression (reply: 8)
1990. Problem with pRb phosphospecific WB - Problem with pRb phosphospecific WB (reply: 3)
1991. SDS-PAGE % - what % gel should I use (reply: 5)
1992. Pronase digestion - (reply: 1)
1993. Problems in SDS PAGE - (reply: 2)
1994. Non specific bands on Western blot - (reply: 1)
1995. Anybody that has used Microcon from Millipore to concentrate protein!? - (reply: 8)
1996. inclusion body had dissolved in 1M urea... - do I have to dialyse it? (reply: 11)
1997. stability of protein sample after in-gel digestion - (reply: 2)
1998. protein purification - (reply: 5)
1999. How to sterilize a protein without denaturating it? - (reply: 2)
2000. mitochondria isolation - sucrose gradient (help, please!) - (reply: 3)
2001. pEt 28-a expression system - (reply: 2)
2002. crude cell lysate - storage of crude cell lysate (reply: 3)
2003. How much dialysis solution? - (reply: 1)
2004. Western Blot of PKCalpha - (reply: 1)
2005. protein precipitation / RNA binding factors - (reply: 3)
2006. protein dialysis - i think i just cant understand it (reply: 15)
2007. enzyme purification - (reply: 3)
2008. colloidal coomassie blue question - (reply: 4)
2009. for protein interaction what lysis buffer I should use? - (reply: 1)
2010. at which step can I store the protein? - purification from inclusion body... (reply: 3)

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