2221. How many antibody should be added for Immunoprecipitation - (reply: 7)
2222. Promegas transcription-translation system - has anyone used? (reply: 2)
2223. Preparing protein Mass Spec samples - (reply: 1)
2224. 38kda protein appearing as smear on western - (reply: 5)
2225. How to stain native proteins on PVDF before immunoblotting - (reply: 3)
2226. Having problem preparing sample for 2D gel. Help pls. - (reply: 2)
2227. sds page of protein in tfa & acotonitrile - (reply: 1)
2228. SDS gel strangely running: curved ladder - Ladder starts running vertical, and then levels horizontal in the running gel (reply: 7)
2229. Western Blotting - Old school - Looking for old protocol (reply: 3)
2230. Protein don't migrate in SDS PAGE - my protein is wierd!! help!! (reply: 1)
2231. Abbrevation for RPMI - (reply: 1)
2232. proteolytic digests - which enzymes cut where.... (reply: 6)
2233. Several questions about Western blotting of tissues - (reply: 2)
2234. protein glycosylation analysis - (reply: 3)
2235. any software can calculate the charge of protein - (reply: 8)
2236. Getting my primary antibody for Western - (reply: 6)
2237. Stripping of nitrocellulose membraned - (reply: 7)
2238. Looking for genomic targets of a DNA binding protein. - (reply: 1)
2239. banging my head against the wall - (reply: 7)
2240. Storage of whole cell lysates - How? (reply: 2)
2241. Questions about proteins Please Help! - (reply: 3)
2242. Accidentally transferred reverse! Help me! - (reply: 3)
2243. Imidazole in column elution - Removing Imadazole from purified protein without dialysis (reply: 1)
2244. Using Lipofectamine - What kind of efficiency do you get? (reply: 6)
2245. How to get DNA binding protein in the clear supernatants? - (reply: 8)
2246. antibody generation - big problem: PLEASE HELP (reply: 1)
2247. Western Blotting protocol-specific questions - (reply: 3)
2248. Trouble with S35 labeling of exogenously expressed proteins - (reply: 1)
2249. Expected protein size ? - fusion protein-size in kDa? (reply: 8)
2250. Gel Filtration - which system is most suitable? - (reply: 3)

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