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2281. What is the best way to identify a protein isoform? - (reply: 4)
2282. western clarification - (reply: 3)
2283. problem purifying a his-tag protein - (reply: 2)
2284. need ideas for protein protein interaction - protein interaction assays (reply: 3)
2285. Unexplained His-tag cleavage - (reply: 2)
2286. FLuorescence methods - (reply: 2)
2287. to prepare westen blot transfer buffer, instead of methanol can i use ethanol. - (reply: 3)
2288. Need SDS purple loading sample dye recipe! - (reply: 3)
2289. Condition for sonication of protein solution to get rid of DNA? - How long to sonicate and with what amplitude? (reply: 4)
2290. Who have used beads covered with protein in ELISA? - Are they heavy enought to be washed out? (reply: 2)
2291. Proteases - need papers (reply: 1)
2292. How much concentration of PEG is useful for protein-protein interaction? - (reply: 1)
2293. How to remove LPS from protein solution - (reply: 2)
2294. protein synthesis - (reply: 2)
2295. Urea gel - preparation of urea gel (reply: 2)
2296. How to conjugate the antibody to the beads. - (reply: 4)
2297. Gelatin zymography, problem with running - (reply: 3)
2298. tricin gel problem - shrinking of stacking gel (reply: 6)
2299. Storing SDS-page gels - (reply: 1)
2300. MDR western - (reply: 1)
2301. Different trend of results between silver staining and coomassie blue staining? - (reply: 9)
2302. DH5 Alpha protein expression - (reply: 1)
2303. Extract hemoglobin from blood for starch gel electrophoresis - (reply: 1)
2304. GST protein purification - only 3% bind to beads (reply: 6)
2305. Contamination after purification - (reply: 1)
2306. Proteins expression-temperature inducible vs IPTG inducible system - (reply: 2)
2307. Best electrophoresis set up? Biorad vs. Hoeffer - (reply: 4)
2308. protein can be found by IHC but not WB - (reply: 2)
2309. Qn on affinity column chromatography - regarding some of the mechanism behind it (reply: 1)
2310. why get rid of imidazole in his tag protein purification - (reply: 3)
2282. western clarification - (reply: 3)
2283. problem purifying a his-tag protein - (reply: 2)
2284. need ideas for protein protein interaction - protein interaction assays (reply: 3)
2285. Unexplained His-tag cleavage - (reply: 2)
2286. FLuorescence methods - (reply: 2)
2287. to prepare westen blot transfer buffer, instead of methanol can i use ethanol. - (reply: 3)
2288. Need SDS purple loading sample dye recipe! - (reply: 3)
2289. Condition for sonication of protein solution to get rid of DNA? - How long to sonicate and with what amplitude? (reply: 4)
2290. Who have used beads covered with protein in ELISA? - Are they heavy enought to be washed out? (reply: 2)
2291. Proteases - need papers (reply: 1)
2292. How much concentration of PEG is useful for protein-protein interaction? - (reply: 1)
2293. How to remove LPS from protein solution - (reply: 2)
2294. protein synthesis - (reply: 2)
2295. Urea gel - preparation of urea gel (reply: 2)
2296. How to conjugate the antibody to the beads. - (reply: 4)
2297. Gelatin zymography, problem with running - (reply: 3)
2298. tricin gel problem - shrinking of stacking gel (reply: 6)
2299. Storing SDS-page gels - (reply: 1)
2300. MDR western - (reply: 1)
2301. Different trend of results between silver staining and coomassie blue staining? - (reply: 9)
2302. DH5 Alpha protein expression - (reply: 1)
2303. Extract hemoglobin from blood for starch gel electrophoresis - (reply: 1)
2304. GST protein purification - only 3% bind to beads (reply: 6)
2305. Contamination after purification - (reply: 1)
2306. Proteins expression-temperature inducible vs IPTG inducible system - (reply: 2)
2307. Best electrophoresis set up? Biorad vs. Hoeffer - (reply: 4)
2308. protein can be found by IHC but not WB - (reply: 2)
2309. Qn on affinity column chromatography - regarding some of the mechanism behind it (reply: 1)
2310. why get rid of imidazole in his tag protein purification - (reply: 3)
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