2611. WB- proteins of lower-than-expected MW - (reply: 1)
2612. Identification of phosphorylation site - help (reply: 1)
2613. which agarose to use for pre-clearing - IP (reply: 1)
2614. shift of protein bands in SDS gel electrophoresis - where does it come from, can it be improved (reply: 2)
2615. staining gels and western blot - (reply: 1)
2616. High protein expression but low protein activity - MBP-tagged fusion protein inactive (reply: 2)
2617. How much a protein converted into a crystal? - crystallization (reply: 2)
2618. Is 100 ug liver tissue protein lysate too much for western blot? - (reply: 2)
2619. Looking for ELISA kit for proteinase 3 - Not anti-proteinase 3 antibody ELISA kit (reply: 1)
2620. Western blot-quantitation - (reply: 4)
2621. Smearing after IP - (reply: 2)
2622. about using BL21(DE3)plys - (reply: 4)
2623. lactate assay - anyone know a good kit? (reply: 2)
2624. How to make pre-stained protein marker in lab - (reply: 1)
2625. Purification of proteins from human tissue - SDS_PAGE (reply: 1)
2626. Problem in using EMSA to assay protein fractions - (reply: 1)
2627. shelf life of PMSF/ leupeptin in buffer - (reply: 1)
2628. No protein expression after IPTG induction - No protein expression after IPTG induction (reply: 6)
2629. DNAse and NaF in lysis buffer...a good idea? - Does NaF or Na3VO4 inactivate DNAse I? (reply: 2)
2630. problem with stripping membranes of western blott - do you know some efficient stripping buffer (reply: 3)
2631. sodium azide as protease inhibitor - protein overexpression (reply: 4)
2632. i let my column dry out by mistake! - (reply: 2)
2633. my protein does not freeze, help - (reply: 11)
2634. BSA purity for Western Blot? - What BSA to use? (reply: 1)
2635. how can I take this non specific band out?? - lysis did not go well (reply: 6)
2636. brain protein isolation for WIB - can I simply use 4xLB+BME? (reply: 2)
2637. lysis buffer----SDS or PMSF? - (reply: 1)
2638. Ammonium Sulphate precipitation - saturation required based on molecular weight (reply: 3)
2639. expression in Pichia - (reply: 1)
2640. Keratin protein - Protocol for lysis (reply: 1)

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