1651. How to do gel filtration - (reply: 3)
1652. problem with SDS-PAGE - (reply: 5)
1653. Protein Precipitation (wessel and Fluege method) - 2 questions (reply: 7)
1654. Small peptides in gel - What to do to see a 2kDa peptide in coomassie gel (reply: 25)
1655. How to get rid of contaminated nucleases from purified proteins - (reply: 2)
1656. Western blot gel running problem - Gel Electrophoresis (reply: 7)
1657. enzyme activity assay - succinate dehydrogenase (reply: 2)
1658. UREA in SDS-PAGE - How to make the gel (reply: 1)
1659. Cleaving a protein from GST - Troubleshooting methods to liberate my protein from gluthathonine sepharose (reply: 3)
1660. calculations of western bands - which software is better? (reply: 3)
1661. protein purification -- 2 or more columns - (reply: 9)
1662. 6% gel westren blot not working as well as 10% - (reply: 1)
1663. Protein precipitates - (reply: 7)
1664. protein folding - GST-fusion protein fluorescence (reply: 2)
1665. what buffer will prevent the precipitation of protein at -20? - (reply: 5)
1666. Use of Urea instead of SDS to denature protein for Western? - (reply: 2)
1667. what is the best method to concentrate protein - (reply: 2)
1668. Protein Purification - (reply: 1)
1669. protein for crystalization - (reply: 1)
1670. IPTG induction - (reply: 2)
1671. I accidentally put two start codons on my protein . . . - (reply: 3)
1672. non-reductive gel to check the disulfide bond - (reply: 14)
1673. How to convert the OD of protein to its concentration? - (reply: 2)
1674. Cell Lysis - (reply: 2)
1675. Supershift - (reply: 1)
1676. problems with Immunoprecipitation- Western Blotting - Rabbit IgG secondary givi - (reply: 1)
1677. hexokinase assay - (reply: 1)
1678. my protein is 18Kda but runs as a 25kda on WB - why ? - (reply: 13)
1679. how to remove media components from protein solutions - (reply: 4)
1680. His-Tag Question (pQE30/31/32 Qiagen Vector) - (reply: 2)

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