151. protein extraction from mammalian cell line - preferred protocol? - (reply: 2)
152. Native & SDS - TOTALLY CONFUSED,kindly help me (reply: 7)
153. Dephosphorylation and IP - (reply: 12)
154. how to find the modification enzyme of a target protein - (reply: 1)
155. Western Blot Blocking - Blocking for 1hr vs overnight (reply: 8)
156. Antifungal Proteins - (reply: 1)
157. Stripping buffer - (reply: 5)
158. TCA precipitation - (reply: 3)
159. Monoclonal vs Polyclonal Abs - (reply: 1)
160. 6XHistaq proteins - (reply: 2)
161. How to lyse mitochondria in order to measue complexIII activity? - (reply: 2)
162. minimum protein conc for immunoprecipitation - what's the minimum needed for results (reply: 1)
163. Precipitate proteins but not 1kDa peptide? - (reply: 3)
164. Can Triton be used for 2D PAGE - Detergent compatibility for 2D PAGE (reply: 1)
165. Protein Quantitation - Methods thats are used for measure the low protein concentation (reply: 9)
166. Protein Extraction from SDS-PAGE - (reply: 1)
167. Question about sodium azide...... - (reply: 3)
168. Comparing Amino Acid Sequences - (reply: 2)
169. Transfer buffer - (reply: 5)
170. do you get consistent results on MALDI? - peptide mass fingerprinting? (reply: 1)
171. How to sterilize Ovalbumin solution? - (reply: 3)
172. Nuclear extract loading control in Western blot - (reply: 3)
173. Identify crosslinked protein with mass spectrometry ? - (reply: 1)
174. Peptide mapping - Protocol for peptide mapping (reply: 2)
175. Checking purity of plasma membrane fraction - (reply: 8)
176. ion exchange chromatography - (reply: 2)
177. positive controls - simple question... whats best??? (reply: 3)
178. Protein A and protein G - (reply: 4)
179. NP40 can be used for lysis nulear membrane? - (reply: 3)
180. Influence of pressure fluctuation in ion exchange column on protein binding, sep - (reply: 2)

© 1999-2008 Protocol Online, All rights reserved.