2491. Help! I am in need of informatiom/a way to obtain a non-fluorescing, color p - (reply: 1)
2492. codon bias? - protein expression (reply: 11)
2493. Serum Preperation for 2D - (reply: 1)
2494. Silly question on His-tagged protein - (reply: 2)
2495. VERY STUPID QUESTION - (reply: 5)
2496. Sonicating vs. homgenizing - (reply: 5)
2497. Streaks appearing between lanes in my western blot - (reply: 3)
2498. Is actin an internal control for nuclear fraction? - (reply: 3)
2499. Appearence of non specific[non 6His tagged] proteins with Ni-NTA purification. - (reply: 16)
2500. Amidoblack staining - (reply: 1)
2501. Purification of enzymatically active protein from PAGE - (reply: 9)
2502. Change Tris for Phosphate buffer cause precipitation - Which buffer can replace Tris? (reply: 3)
2503. Cell breakage - (reply: 1)
2504. Inhibition Study for Beta-galactosidase - (reply: 1)
2505. disulfide bonds - (reply: 1)
2506. Biotynylation of protein - How better to remove unreacted biotin? (reply: 1)
2507. Sample prep. compatibility with IEF for 2DE - (reply: 1)
2508. Protein rofolding problem - percipitation (reply: 4)
2509. Glycerol gradients - glycerol gradients for cell extract fractionation (reply: 2)
2510. U-shaped smear on EMSA gel - (reply: 1)
2511. sonication free protein extraction... - (reply: 2)
2512. BSA,PEG8000 and Tween20 - (reply: 1)
2513. Glycosylation - (reply: 1)
2514. How to stain /destain quickly SDS-PAGE gel? - your patents? (reply: 13)
2515. STOP codon preference for mammalian expression - Which STOP codon to use! (reply: 1)
2516. Effect of APS (Ammonium persulfate) to protein - (reply: 1)
2517. Why use ammonium bicarbonate? - (reply: 4)
2518. cell fractionation plus IP - (reply: 12)
2519. Preparation of Sodium Vanadate. - (reply: 1)
2520. Protein precipitation problems - (reply: 3)

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