2671. WB for large proteins - Transfer conditions with PVDF membranes (reply: 5)
2672. WB: one single band but at the wrong size... - what to make of it? (reply: 4)
2673. quantify bands - (reply: 4)
2674. about cell sonication - (reply: 2)
2675. Acetyl coenzyme A and dialysis - Removing AcCoA and dialysis problem (reply: 2)
2676. TCA precipitation - (reply: 6)
2677. Western blot of phosphoproteins - (reply: 2)
2678. E coli die after inducer - (reply: 3)
2679. Help: anyone who has co-transfected TWO different plasmids - - how much of each DNA to use? (reply: 5)
2680. Protein Expression - Question (reply: 1)
2681. denature a protein of interest - (reply: 4)
2682. Supershift/EMSA problem - (reply: 1)
2683. questions about mass spectrometry and cell culture - (reply: 1)
2684. Pippeting nickel-agarose resin - - OK to use a P200 pippete tip? (reply: 5)
2685. Lysis buffer for membrane protien - (reply: 1)
2686. aspecific bands in cellculture protein, not in tissue - sds page (reply: 3)
2687. Co IP with myc and ha tagged fusion proteins - (reply: 7)
2688. Protein interaction - (reply: 3)
2689. uneven western blot - (reply: 1)
2690. Plasmid - Choosing a plasmid (reply: 2)
2691. Stability of protein in powdered leaf sample - Protein stability after quick freeze in liquid N2 (reply: 1)
2692. PVDF vs. nitrocellulose membrane - (reply: 4)
2693. problem with Protein concentration determination and westernblot - (reply: 11)
2694. Protein not penetrating the resolving part of the gel - (reply: 2)
2695. BCA assay - folded or denatured protein (reply: 2)
2696. The composition of Elution buffer - I want to know what is the composition of your buf (reply: 1)
2697. how to prove a protein-protein interaction is direct? - (reply: 5)
2698. Imidazole reduction causes precipitation - protein purification (reply: 2)
2699. Stripping membranes for WB - To strip or not to strip... ? (reply: 9)
2700. Effect of methanol in Westen transfer buffer - help (reply: 2)

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