331. IP:reducing or non-reducing - (reply: 1)
332. Horizontal Vs. vertical IEF…? - which one is better (reply: 1)
333. reuse of mineral oil in 2 D gel electrophoresis - can we reuse mineral oil for 2 D gel electrophoresis (reply: 1)
334. Diluting/mixing of antibodies - exact method? - (reply: 1)
335. Safe disposal of powder acrlamide - (reply: 2)
336. Snail Enzyme - Cellulase, xylanase, reducing sugar (reply: 1)
337. resolving 27 kDa protein - (reply: 3)
338. Myosine Light chain WB - (reply: 4)
339. how to insert a thrombin cleavage site - ....into my vector (reply: 3)
340. BSA dilutions for micro-bradford - (reply: 4)
341. help optimize an western blot for 6kd protein - western blot tranfer problem (reply: 16)
342. Crossreactivity or protein complex under denaturing conditions? - (reply: 2)
343. Killing bacteria without denaturing proteins - (reply: 9)
344. TYROSINASE ACTIVITY ASSAY - WHO KNOW PROTOCOL? (reply: 2)
345. RIPA Buffer and DNA - (reply: 3)
346. Phosphoprotein staining? - (reply: 5)
347. Qiagen penta-HIS antibody not reusable - looses activity after freeze-thaw-cycles (reply: 2)
348. My enzyme is not active :( - (reply: 3)
349. Polyacrylamide gels shriveling up during transfer - (reply: 1)
350. emsa oligo annealing - biotin labelled oligos (reply: 5)
351. What is FRET state? - (reply: 2)
352. Simple Bradford Assay Question - water dilutions - (reply: 5)
353. PROTOCOL FOR ALBUMIN REMOVAL - (reply: 2)
354. emsa competition oligos - how to make up competition oligos (reply: 3)
355. Coating buffer for ELISA - Buffer composition needed...Help (reply: 2)
356. seeking TCA precipitation advice - (reply: 2)
357. WB reverse image solution?! - white band on darck background (reply: 2)
358. Fluorescent detection of western blots - (reply: 3)
359. protein size determination? - (reply: 33)
360. Buffer for cation exchange chromatography - acetic acid or sodium acetate? - (reply: 1)

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