1801. problem in my gluthatione seharose beads? - (reply: 15)
1802. HA affinity column - for activity assay (reply: 2)
1803. why can't I get GST-fusion protein? - (reply: 2)
1804. Storing proteins - (reply: 2)
1805. is tissue from rat kidney reacting with ECL? - (reply: 9)
1806. SDS sample buffer and serum - why is it solidifying? (reply: 3)
1807. Please recommend some solutions for size-exclusion chromatography - (reply: 4)
1808. Rhinovirus (RV) GEL FILTRATION - (reply: 1)
1809. prediction of protein phosphorylation sites, how? - (reply: 4)
1810. just a word of advice - better to lean from others mistakes. ECL and missing bands (reply: 1)
1811. Storage of GST proteins in crude lysates - (reply: 1)
1812. silly question by an FPLC virgin - (reply: 5)
1813. problem with the size of protein - (reply: 4)
1814. iNOS and nNOS protein quantification from rat tissue homogenate - (reply: 1)
1815. Western after Ip- no IP protein, but .... - (reply: 1)
1816. dmp coupling to Protein G beads - (reply: 4)
1817. immunoprecipitation of nuclear protein - (reply: 2)
1818. His-tagged proteins - bacteria or mammalian systems? - Which is better and why? (reply: 1)
1819. Please help me with bradford assays - (reply: 5)
1820. Cell line to be used for protein expression an purification - (reply: 8)
1821. Western Blot - membrane-bound protein boiling step - (reply: 1)
1822. Wet transfer vs Semi-dry transfer - (reply: 2)
1823. Storing gels . . . - (reply: 3)
1824. Protein expression problem - coexpression? (reply: 1)
1825. Protein Full Names - (reply: 3)
1826. Human IgA column - (reply: 1)
1827. Ponceau S as loading control on Westerns? - (reply: 3)
1828. protein samples on ice - how long is too long? (reply: 3)
1829. His tag protein purification problem - Binding of undesired protein (reply: 6)
1830. Colony lift followed by probing with a peptide; does this exist? - (reply: 6)

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