RNA polymerase a sa fractionation control? - (Jul/31/2007 )

I've just read a paper where they have used RNA pol. as a fractionation control in an immunoblot, I was wondering if someone can explain the idea behind it?

-smoochiepie79-

QUOTE (smoochiepie79 @ Jul 31 2007, 04:30 PM)

I've just read a paper where they have used RNA pol. as a fractionation control in an immunoblot, I was wondering if someone can explain the idea behind it?

Nevermind, got it:P

-smoochiepie79-

maybe cytoplasmic / nuclear separation ?

-fred_33-

QUOTE (fred_33 @ Jul 31 2007, 04:58 PM)

maybe cytoplasmic / nuclear separation ?

Yes, it is used as a cellular control when fractionating supernatants from whole cells from a bacterial culture:).

-smoochiepie79-