361. E.coli transformation - (reply: 1)
362. Can IP shift the MW of a protein? - (reply: 2)
363. Preparation of lysis buffer for enzyme extraction - (reply: 5)
364. Histrap or Ni-NTA superflow? - (reply: 2)
365. beta galactosidase assay - (reply: 1)
366. How to tackle the problem of protein denaturation while storing? - (reply: 10)
367. anti-gfp antibodies - (reply: 4)
368. Cation exchange column - which is the best one? - (reply: 1)
369. GFP western - (reply: 3)
370. problems with zymography gels - (reply: 1)
371. protein expressed once,but stopped expressing then - (reply: 1)
372. A question about loading control - (reply: 2)
373. Sephadex G-50 gel filtration chromatography - (reply: 1)
374. native gel problem - (reply: 7)
375. post-translational modifications which increase molecular weight of the protein - (reply: 7)
376. Isolation of a clean cytoplasmic fraction - (reply: 2)
377. o-NPG assay - (reply: 2)
378. nonreducing condition - (reply: 2)
379. How to get clean negative result in immunoprecipitaion? - (reply: 2)
380. sds page (gel preparation) - (reply: 4)
381. Extraction of protein from inclusion bodies - (reply: 1)
382. Dialysis ? - (reply: 1)
383. Agent to punch hole in cell membranes - (reply: 1)
384. Isolation of golgi transmembrane protein - (reply: 4)
385. protein nature in different pH? - (reply: 3)
386. Concentrating Supernatant - (reply: 2)
387. cell lysate for immunoprecipitation - (reply: 2)
388. protein determination in loading buffer - (reply: 1)
389. Collagen IV purification - (reply: 2)
390. Brain Microsomes Again :( - (reply: 4)

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