601. HPLC acetone Precipitation SDS-PAGE not Working - (reply: 1)
602. Why is resistance increasing with SDS PAGE gel run - (reply: 4)
603. washing buffer retained in Ni-NTA column after centrifugation - (reply: 5)
604. GST pull-down:Is there any way to reduce nonspecific binding of protein to gluta - (reply: 3)
605. Please help me with the western blot problem - (reply: 2)
606. Protein G beads for IP - (reply: 1)
607. How to reduce the dimers - (reply: 3)
608. Storing a NC membrane that has yet to be processed - (reply: 2)
609. no band in 6% gel - (reply: 4)
610. Gel shift on formalin tissues? - EMSA (reply: 1)
611. lysis buffer for coip with a membrane protein - (reply: 2)
612. Tris Tricine Gel Running Buffer - (reply: 1)
613. Problem in purifying fusion protein with His Tag - Fusion protein found in Flow Through (reply: 6)
614. How to concentrate my small peptide - (reply: 7)
615. Band sizes after co-IP - (reply: 4)
616. Co-immunoprecipitation of phospho-proteins - I"m looking for a sensitive protocol to maintain the phosphorylate (reply: 2)
617. Is it OK to use monoclonal antibody against its host protein for westen - (reply: 4)
618. Bacterial Stock - (reply: 4)
619. Western Blot: Re-use of membrane? - (reply: 4)
620. problem in centrifuge of protein - (reply: 3)
621. How many His residues? - (reply: 1)
622. T7 promoter strength - (reply: 2)
623. for protein refolding - (reply: 5)
624. WB trasfer problem - (reply: 11)
625. protein kinase C (PKC) inhibitors - (reply: 1)
626. Size exclusion chromatography for 23 and 20.5KDa? - protein purification (reply: 3)
627. Protein extraction using trizol - (reply: 2)
628. Fixation on Acrylamide - Fixing a Peptide on Bio-Rad Tris/Tricine SDS-PAGE (reply: 6)
629. How to chose vector and E.coli strain for protein expression - (reply: 2)
630. Did my Ab provider do me wrong? - Judging the purity of my antibody (reply: 3)

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