1201. How many times can one strip a membrane? - (reply: 2)
1202. SDS PAGE Help - (reply: 3)
1203. Kinase - Phosphorylation of Kinases (reply: 1)
1204. Can one keep NC menbrane overnight and do ECL the next day? - (reply: 4)
1205. how larg should my tissue sample be for western blotting? - (reply: 1)
1206. smearing at top of resolving gel - SDS-PAGE (reply: 1)
1207. Blotting gel after Bio-Rad Coomassie staining? - (reply: 5)
1208. specific ser/Thr phosphorylation detection by in house antibody - (reply: 1)
1209. Silver Staining Principle and protocol - (reply: 2)
1210. Strange phenomenon with western blot - Double bands (reply: 2)
1211. MMP9 protein zymography - (reply: 5)
1212. bacterial protease expression - expression (reply: 5)
1213. NIH ImageJ for Immunoquantitation - guide? (reply: 1)
1214. Bl21 plys expression system - protocol (reply: 7)
1215. how to isolate non-crosslinked protein? - (reply: 6)
1216. loading control in IP? - IP (reply: 3)
1217. Co-IP..... - What's important for the lysis buffer/washing buffer? (reply: 2)
1218. sp-cation exchange column.. - phosphate /tris buffer ph 7.0 (reply: 2)
1219. analytical IEC for basic protein - pI 9.4.. for a start? (reply: 10)
1220. The Molarity of 1X PBS - Do anyone know the Molarity of 1X PBS? (reply: 1)
1221. Ethanol precipitation of protein sample - What is the protocol? (reply: 4)
1222. recombinant protein - does GST affect the function? (reply: 11)
1223. Recombinant IL-2 proteins.. - Work for cancer.. how? (reply: 1)
1224. Nuclease vaccine.. - What possible prevention wud it help? (reply: 1)
1225. Types of aggregates in a protein.. Disulphide linked? Hydrophobicity related? - Conditions that can make or break them? (reply: 5)
1226. the plasmid even smaller than the empty PET-22b vector! - the plasmid even smaller than the empty PET-22b vector! (reply: 1)
1227. How to purify a sticky protein - (reply: 1)
1228. phosphoprotein detection - (reply: 8)
1229. Reasons for wrong protein size on Western blot and how to deal with - (reply: 8)
1230. Rabbit skin collagen - MW? (reply: 5)

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