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1081. question about lysis of cells - centrifuge or not--important? (reply: 1)
1082. ACID-PAGE vs SDS-PAGE - Does anyone have experience with this? (reply: 2)
1083. Ultrasonic bath - bacteria lysis using a ultrasonic bath (reply: 7)
1084. Bacterial strains used in protein expression - (reply: 5)
1085. Protein high molecular weight standards for gel filtration - (reply: 2)
1086. How many methods can demenstrate to endogenous protein interactio? - (reply: 3)
1087. Lyse cells with TCA - (reply: 1)
1088. gst tagged protein expresion - (reply: 3)
1089. urea - (reply: 9)
1090. no bands in sds page - (reply: 2)
1091. Anybody has a good phosphorylated IKKa antibody? - (reply: 3)
1092. Larger protein size in SDS gel - why is it like tat? (reply: 11)
1093. will freeze thaw cycles or sonication disrupt protein interaction? - (reply: 3)
1094. Purification of GST-fusion - GST-fusion protein: How to express and purify (reply: 7)
1095. Additional bands on SDS-PAGE under reducing conditions - (reply: 1)
1096. Antibody Dilution - (reply: 10)
1097. Protein pellet won't resuspend in buffer following acetone precipitation - (reply: 4)
1098. A CoIP problem - How to reduce the non-specific binding of Protein A beads? (reply: 4)
1099. Resolve High Molecular Weight Proteins in SDS-PAGE - (reply: 3)
1100. SDS-PAGE hi pH/hi Tris loading buffer runs funny - Do proteins generally run funny on Lammelli SDS-PAGE with high pH/high (reply: 1)
1101. Help NO SIGNAL WITH WESTERNS - (reply: 4)
1102. Storing a western blot over the weekend - (reply: 4)
1103. Help needed with western blot with human serum - (reply: 2)
1104. expression with M15 - Any special tips?? (reply: 3)
1105. rapid translation system - any good product? (reply: 1)
1106. Caspase-3 Identification - (reply: 3)
1107. native protein - (reply: 2)
1108. concentration of protein in urea - I need help to concentrate proteins (by precipitation?) that are in 8M (reply: 5)
1109. scanner for quantification of western bands - (reply: 1)
1110. FPLC: Gel Filtraito - Running Standards, Triton X-100, Mitochondria on a column (reply: 3)
1082. ACID-PAGE vs SDS-PAGE - Does anyone have experience with this? (reply: 2)
1083. Ultrasonic bath - bacteria lysis using a ultrasonic bath (reply: 7)
1084. Bacterial strains used in protein expression - (reply: 5)
1085. Protein high molecular weight standards for gel filtration - (reply: 2)
1086. How many methods can demenstrate to endogenous protein interactio? - (reply: 3)
1087. Lyse cells with TCA - (reply: 1)
1088. gst tagged protein expresion - (reply: 3)
1089. urea - (reply: 9)
1090. no bands in sds page - (reply: 2)
1091. Anybody has a good phosphorylated IKKa antibody? - (reply: 3)
1092. Larger protein size in SDS gel - why is it like tat? (reply: 11)
1093. will freeze thaw cycles or sonication disrupt protein interaction? - (reply: 3)
1094. Purification of GST-fusion - GST-fusion protein: How to express and purify (reply: 7)
1095. Additional bands on SDS-PAGE under reducing conditions - (reply: 1)
1096. Antibody Dilution - (reply: 10)
1097. Protein pellet won't resuspend in buffer following acetone precipitation - (reply: 4)
1098. A CoIP problem - How to reduce the non-specific binding of Protein A beads? (reply: 4)
1099. Resolve High Molecular Weight Proteins in SDS-PAGE - (reply: 3)
1100. SDS-PAGE hi pH/hi Tris loading buffer runs funny - Do proteins generally run funny on Lammelli SDS-PAGE with high pH/high (reply: 1)
1101. Help NO SIGNAL WITH WESTERNS - (reply: 4)
1102. Storing a western blot over the weekend - (reply: 4)
1103. Help needed with western blot with human serum - (reply: 2)
1104. expression with M15 - Any special tips?? (reply: 3)
1105. rapid translation system - any good product? (reply: 1)
1106. Caspase-3 Identification - (reply: 3)
1107. native protein - (reply: 2)
1108. concentration of protein in urea - I need help to concentrate proteins (by precipitation?) that are in 8M (reply: 5)
1109. scanner for quantification of western bands - (reply: 1)
1110. FPLC: Gel Filtraito - Running Standards, Triton X-100, Mitochondria on a column (reply: 3)
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