2551. Large scale protein purification - Yellow and cyan fluorescent protein (reply: 4)
2552. Western background - (reply: 2)
2553. loading buffer concentration - (reply: 2)
2554. protein purification - (reply: 3)
2555. peptide expression - (reply: 3)
2556. optomizing IP results - what works for you? (reply: 1)
2557. Staining SDS-PAGE prior to transfer - (reply: 1)
2558. Does imidazole absorbe at 280 nm? - How do you measure your protein content? (reply: 4)
2559. chemiluminescent substrate - western blotting (reply: 2)
2560. Western background - vertical stripes ! urgent - (reply: 3)
2561. How to make western results clean??? - (reply: 3)
2562. background in western - very strange stripes ?! - (reply: 2)
2563. How much 3D structure data is available to date? - (reply: 1)
2564. Troubleshooting flag-tagged secreting protein expression - Troubleshooting, Improvement, tricks! (reply: 5)
2565. Extremely lunatic competent cell - transformation (reply: 11)
2566. Proteinase K activation/preparation - water or buffer in proteinase K (reply: 3)
2567. buffer exchange - (reply: 1)
2568. the range of protein amount for loading - western blotting (reply: 4)
2569. peptide mapping - (reply: 6)
2570. Glycine in gel; fiber typing - (reply: 1)
2571. No straight band-WESTERN BLOTTING - (reply: 1)
2572. Incubating the filters two overnights with primary antibody. - WESTERN BLOTTING (reply: 3)
2573. Protein measuring - (reply: 3)
2574. Lost all of the signals after stripping - WESTERN BLOTTING (reply: 5)
2575. optimal SDS separation protein - (reply: 3)
2576. Help!recombinant Protein degradation during purification - Proteolysis (reply: 1)
2577. Western blotting - (reply: 2)
2578. extraction of membrane protiens - protocol for extraction of membrane bound protein? (reply: 6)
2579. Detergent Concentrations for Co-IP - (reply: 2)
2580. Strange SDS-PAGE result - (reply: 12)

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