61. need help - (reply: 1)
62. DsRed stable transfections? - problems with Stoplight vector in Flip-in cells (reply: 4)
63. Protein Extraction and Centrifugation - (reply: 1)
64. Western: bands spreading out? - (reply: 1)
65. Purification of gst and His tagged protein - after purification there are still many contaminated bands (reply: 1)
66. Protein purification? - urgent (reply: 3)
67. MS/MS - Difference b/w MS/MS and Maldi-TOF (reply: 1)
68. precipitation of protein with acetone - (reply: 3)
69. question about the protein structure and function - (reply: 1)
70. How to purify unknown protein? - (reply: 2)
71. protease inhibitor, TRI reagent and western - (reply: 2)
72. How to determine immunogobulins from milk? - (reply: 3)
73. wobbly bands in WB - (reply: 3)
74. Proteins recover from different buffer - How to obtain again proteins from ST buffer? (reply: 2)
75. Advantages of Proteomics Study - (reply: 1)
76. Autoclaving nuclei isolation buffer - (reply: 8)
77. Non-specific binding to immunoprecipitation beads - what to do to minimize it? (reply: 2)
78. Ni-NTA elute without imidiazole under native condition - (reply: 4)
79. Immunoprecipitation wash + urea? - Isn't it too strong? (reply: 2)
80. problem dissolving protein pellet after aceton precipitation - (reply: 3)
81. a question in protein dialysis - protein dialysis (reply: 1)
82. How to make gradient gel for western blot ? - (reply: 8)
83. Why 150 mM KCL in homogenization buffer - (reply: 7)
84. RIPA buffer - problems (reply: 1)
85. westerm Blot membrane - (reply: 4)
86. 8M urea buffer for protein extraction - (reply: 6)
87. Buffer storage question - (reply: 2)
88. SDS Gel for 2D -DIGE - Problems having SDS Page to polymerize (reply: 3)
89. how to improve protein expression - (reply: 2)
90. Capture of silver stained gels - (reply: 1)

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